[Histonet] Alk Phos Crystals
Melanie Black
ctsblack <@t> capeheart.uct.ac.za
Tue May 3 05:59:27 CDT 2005
Hi There
Can anybody shed some light onto the subject of crystals forming
after the use of BCIP/NBT on alk phos staied tissue. At first when I
mount the slides, you do not see the precipitate, btu within hours,
it deveopes. I must add that the tissue is ZINC fixed. As zinc is a
precippitant, I am not sure where to look for the problem. DAKO has
no idea!!
This problem has been discussed before, but I cannot recall if it was
ever resolved.
Many Thanks
Melanie Black
--
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Observatory
7925
Republic of South Africa
Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935
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