[Histonet] Ki67 - kidney.

[Kelly D Mcqueeney]

Hi Christian,
I have used rabbit polyclonal human Ki67 antibody on rat kidney and it 
worked beautifully.  Make sure you use a wash buffer with tween-20. 
Simply follow the protocol recommended by Abcam (ab833). I used heat 
antigen retrieval in citrate buffer (Dako), 3% BSA (only) as a block and 
Envision+ rabbit kit (Dako) rather than ABC. The incubation time is O/N 
at 4C.

Let me know if you need more detailed information. You can also use Ki67 
antibody from Santa Cruz.

Good luck,
Kelly

christian <@t> eisbo.dk wrote:

>Hey users of histonet.
>I am trying to stain rat kidneys for Ki67!
>I have bought the recomended antibody. But this one is for human!
>Do anyone know if this can be used since i dont have a outcome!
>Do anyone have a protocol that works?
>Christian Eisbo
>
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