[Histonet] CD2

[Donovan, Mark]

Elizabeth,
 
I have just been playing with a sample of CD2 antibody from Novocastra,
Clone AB75, Cat NCL-CD2-271. 
Results have been very good on our routine FFPE tonsil controls using HIER
Tris-EDTA pH 8 (Microwave Pressure Cooker) 3 min, 30 min primary incubation,
Chemicon Select LSAB detection with Dako DAB+ chromogen. Beautiful membrane
staining across a dilution range from 1 in 50 to 1 in 400. Staining was done
using the Lab Vision Autostainer. 
An antibody worth looking at from what I have seen so far.
 
Regards,
 
Mark Donovan
The Alfred Hospital
Melbourne, Australia
 
Original message
 
Message: 10
Date: Thu, 31 Mar 2005 10:03:35 -0700
From: "Earle, Elizabeth" <eearle <@t> abrazohealth.com>
Subject: [Histonet] CD2
To: <histonet <@t> lists.utsouthwestern.edu>
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      <4727B24D9A8575479222635B57E3B3DBCB1EB8 <@t> mail.vhswest.local>
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Does anyone know of a CD2 that works in FFPE tissue?
 
Thanks
 
E Earle
 
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