[Histonet] Immunofluorescence

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Hello,all,
I am doing immunofluorescence in bone sections, but the results are not good, I do not know the reasons, can anybody do me a favor?
 
My questions:
1. In bone sections, I found that some positions are stained, but some 
positions are not stained, I do not know why it appears. 
2. In addition, this protein should be expressed strongly in bone 
marrow, but sometimes it is very weak, sometimes it is not stained. why?
 
Thank you!
 
Guofeng




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