[Histonet] Frozen Spleen w/bubbles

[Robyn Vazquez]

Jo Dee,
Are you using a cryogun?  That could be a cultprit.  Also, are putting the slides directly into your fixative?  What kind of stainer are you using?  If your using a chain, put it in the heated section for 5 to 30 seconds.  I used to have this problem and when we put it in the heated section for just a few seconds>30sec that solved the problem.  OR you could have a bad lot of slides.

Robyn
OHSU

>>> Jo Dee Fish <jfish <@t> gladstone.ucsf.edu> 03/30/05 2:30 PM >>>
Hello histonetters,

I am cryosectioning unfixed fresh frozen spleens and when they are 
sectioned I can see a billion bubbles under the section.  Can anyone 
explain-

1.  What is happening?
2.  How can I avoid them?
3.  Is there a fixation procedure that can help?

The sections are falling off of the slide during staining.
Thank you for your help,
Jo Dee
********************************************************************** 
**********

Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-5766
Fax: (415) 355-0230
E-mail: jfish <@t> gladstone.ucsf.edu 

Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA   94158

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