[Histonet] Cryosectioning for LMD POL Slides

[Kristen Broomall]

One of the other techs here is cutting snap frozen muscle for LMD and is
having quite a time getting the sections to pick up on the POL membrane of
the slide. She's tried warming the membrane with her finger, and I'm sure a
couple other tricks, but barely any tissue with stick to it. Certainly not
enough to constitute a good section.

We're using Leica POL Membrane, metal framed slides.

Any suggestions?

Thanks in advance!

Kristen Broomall, HT (ASCP)
A.I. DuPont Hospital for Children
kbroomal <@t> nemours.org

> NOTICE...This electronic transmission is intended only for the person(s)
> named.  It may contain information that is (i) proprietary to the sender,
> and/or (ii) privileged, confidential and/or otherwise exempt from
> disclosure under applicable State and Federal law, including, but not
> limited to, privacy standards imposed pursuant to the federal Health
> Insurance Portability and Accountability Act of 1996 (HIPAA).  Receipt by
> anyone other than the named recipient(s) is not a waiver of any applicable
> privilege.  If you received this confidential communication in error,
> please notify the sender immediately by reply e-mail message and
> permanently delete the original message from your system.
> 
> 
>