[Histonet] Immunofluorescence in bone sections

Wed Mar 30 11:42:34 CST 2005

Hello,all,
I am doing immunofluorescence in bone sections, but the results are not good, I do not know the reasons, can anybody do me a favor?

My questions:
1. In bone sections, I found that some positions are stained, but some positions are not stained, I do not know why it appears. 
2. In addition, this protein should be expressed strongly in bone marrow, but sometimes it is very weak, sometimes it is not stained. why?

Thank you!

Guofeng

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