[Histonet] gelatin embedding[Scanned]

[Kemlo Rogerson]

Make up gelatine:	Gelatine 16g
			Glycerine 15 ml
			Dist water 70 ml
			Thymol 1 crystal
			Heat gelatine in warm water (not too hot) until it
dissolves, add glycerine then thymol when cool. 

Fix tissue in 10 per cent formalin (buffered) then wash in running tap water
overnight (the fixative will fix the gelatine otherwise), place in fresh
gelatine/ glycerine mixture at 37 degrees for 6 hours. Finally place in
fresh gelatine/ glycerine mixture and cool in refrigerator. Trim block fix
in 10 per cent buffered formalin overnight and store in fixative.

Cut sections on a freezing microtome; really good fun.  I used to enjoy
fiddling with these things as a pup.

-----Original Message-----
From: Arvind Pundir [mailto:arvind <@t> nbrc.res.in] 
Sent: 30 March 2005 12:39
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] gelatin embedding[Scanned]

 

	can any one please give detail of how to embed tissue block in
gelatin
	 
	arvind singh pundir
	 NBRC ,
	Deptt. of Neuroanatomy,
	 Haryana,
	INDIA