[Histonet] Pneumocystis carnii staining

[Sandy Cheasty]

Karen,
	We recently had the same problem and solved it using the SIGMA Meth
Silver Kit but used Chromic Acid instead of periodic acid. (We microwave the
meth-silver step) Delicate, clear, reliable staining with zero back ground. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anita dudley
Sent: Tuesday, March 29, 2005 11:16 AM
To: karenharmon <@t> comcast.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Pneumocystis carnii staining

karen, in our lab we have found that we have to over stain the gms to get 
the pneumo to demonstrate.  hope this helps.  anita dudley, providence hosp.

  mobile alabama

>From: "Karen Harmon" <karenharmon <@t> comcast.net>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Pneumocystis carnii staining
>Date: Mon, 28 Mar 2005 16:25:33 -0600
>
>We use a GMS stain to demonstrate pneuomcyctis with successful results 
>about 50% of the time.   We have taken this procedure apart step by step, 
>solution by solution and are at our wits end to determine the cause for 
>failure.  I can assure you that over the past 3 weeks, there is no variable

>that we have not tried.    The ironic part is we never have a problem  
>demonstrating fungi with the GMS, in fact, we never had any problems at all

>with other silver procedures.
>
>Can anyone help with our hit and miss problem with pneumocystis?
>
>Karen Harmon
>HT (ASCP)
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