[Histonet] Target retrieval solution

[Jan Shivers]

In my experience, the time in Target Retrieval solution (of any type/brand)
varies, depending upon the difficulty of exposing specific antigenic sites.
Each antigen will need to be worked up individually, by comparing staining
results of different time lengths in HIER with TR.

I run mine at 10, 15, or 20 minutes.. depending upon the antigen in
question, if you want a general answer.

Jan Shivers
U of MN

----- Original Message ----- 
From: "Larry Woody" <slappycraw <@t> yahoo.com>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, March 29, 2005 10:26 AM
Subject: [Histonet] Target retrieval solution


> Dako makes a 10x target retrieval solution that a lot of people use in the
decloaker but I've heard so many different times being used; ie; 20 min, 10
min, 2 min. or does it matter? suggestions. Larry W.
>
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