[Histonet] CD1a

Richard Cartun Rcartun <@t> harthosp.org
Thu Mar 24 14:33:12 CST 2005

Hi Linda:

A 1:10 dilution sounds "very expensive"!


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu> 03/24/05 03:10PM >>>

We use Biocare's CD1a at 1:10 with 3" HIER in Biocare's decloaking
chamber in Biocare's BORG retrieval buffer and incubate for 32" OR use
mild CC1 cell conditioner on Ventana's Benchmark or Benchmark XT
immunostainers again with a 32" incubation.  Hope this helps.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Nancy
Sent: Thursday, March 24, 2005 1:42 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] CD1a

Hello Histonetters,

Do any of you have suggestions for staining Langerhans cells in FFPE
skin?  I have been wrestling with CD1a and have not had great success.
I would cherish any words of wisdom.


Nancy Lemke

Asterand, Inc.

TechOne Suite 501

440 Burroughs

Detroit, MI  48202


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