[Histonet] H&E slides for HT practical exam

pmarcum <@t> vet.upenn.edu pmarcum <@t> vet.upenn.edu
Wed Mar 23 08:19:39 CST 2005

I agree with Peggy.  It is better to learn to be flexible and 
do excellent work than adhere to policy that only produces a ridgid stain
The goal is to produce slides that are of the highest quality for each tissue
and stain.

Unforutnately we have seen and heard from people taking the test for ASCP
in Histology who cut the same blocks and stianed the slides at the same time
when one passed
and one failed.  I have yet to see anyone on the grading committee address this

Sorry to open this can of worms again however, when I took my test in the "70s I
was told
not to submit any animal tissue (I was doing research on animals only) or I
would fail.  I know
that has changed and I did find a way (pre-HIPPA) to get my tissue and stain but
it shows the differences 
in how we are treated and graded over the years.  Other than what you stated 
for H&E is there a true
standard for each stain or does it depend on the person grading and what they
like or see.

Pam Marcum

Quoting lpwenk <@t> sbcglobal.net:

> Slides can be stained all together or one at a time. There is no way anyone
> can tell.
> In fact, time in each solution can be different for each tissue stained.
> What is important is that EACH slide be well stained - good crisp nuclei
> with chromatin pattern, a minimum of three shades of eosin that does not
> overwhelm the hematoxylin, etc..
> If that takes taking each slide through the different solutions/stains at
> different time intervals, repeating and tweaking the stain  20 times with
> different time intervals from what you normally do, and changing to
> different times for each block depending upon the tissue/length of time it
> has been fixed/tissue thickness/etc. - then do it. It is more much more
> important to know what to do to produce a well stained slide, than to
> rigidly adhere to the same steps/times.
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
> ----- Original Message -----
> From: "Mark Adam Tarango" <marktarango <@t> earthlink.net>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, March 22, 2005 3:52 PM
> Subject: [Histonet] H&E slides for HT practical exam
> > Hi everyone.  I have a co-worker who insists that she must stain all her
> H&E and slides for her practical exam together at once.  My supervisor and I
> haven't ever heard this, and I'm hoping someone can give a reply as to
> whether or not this is something that is required.  She is conviced that if
> she doesn't stain them all together (her H&E slides), someone will know and
> fail her again.    If Peggy Wenk or Jennifer MacDonald are watching, we'd
> like to her from you especially.
> >
> >
> > Thanks,
> >
> >
> > Mark Tarango
> >
> >
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