[Histonet] (no subject)

[Margaryan, Naira]

Hello, all,

 

I am going to do Immunostaining of Frozen Sections .

I have some questions:

1. What to do with slides after remove them from --80 o C freezer?

2. How long the slides have to come to room temperature?

3. How the OCT compound has to be dissolved?

4. What is general protocol for frozen sections?

5. Am I have to apply Avidin/biotin?

 

 

My appreciation for any suggestion,

Naira

 

Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594

nmargaryan <@t> childrensmemorial.org
<mailto:nmargaryan <@t> childrensmemorial.org> 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 



The contents of this e-mail message and any attachments are private and confidential communications intended solely for the addressee(s) named in this message. If you are not the intended recipient of this message, please 1) immediately notify the sender by reply e-mail and then delete this message and its attachments and 2) do not read, use, distribute disclose or copy this message and/or any attachments.