[Histonet] decalcification of avian hock joints

[Ahmed, T (Tanni)]

Dear Histonetters,
 
I am looking for a workable decalcification procedure for decalcifying
samples of avian hock joints (fixed with 10% buffered formalin). I have
used Surgipath's decal 1 solution- placing the embedded blocks on soaked
tissue paper for 30 mins..is this the normal length of time?  Any
comments/suggestions would be gratefully appreciated.
 
Thanks everso,
 
Tanni
 
 

Tanni  S Ahmed

Histopathology, R&D

 

Intervet UK Ltd.

Walton Manor, 

Walton, Milton Keynes,

Buckinghamshire, UK

 

E-mail: tanni.ahmed <@t> intervet.com

 

 

 

 

 

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