[Histonet] Immunofluorescence in paraffin sections
gcallis <@t> montana.edu
Mon Mar 21 11:18:01 CST 2005
Goat is not the only host for seconday antibodies. You could use Donkey
antimouse - FITC. Try Jackson Immunoreasearch for other antibodies that
will NOT cross react. Jackson has a website.
At 09:21 AM 3/21/2005, you wrote:
>I am doing immunofluorescence in paraffin sections. I have some
>difficulties in it:
>1: For pretreatment of tissue sections, I plan to incubate sections with
>trypsin solution, but I do not know :what kind of concentration should I
>choose ? (0.01% trypsin, 0.05% or o.1%)how long does it should last?
>2: Recently, I read a protocol about double immunofluorescence, it shows
>:Antibodies derived from different animal can be mixed and incubated as a
>cocktail (example: rabbit anti-A and mouse anti-B). The same is valid for
>secondary antibodies (example: goat anti-rabbit Texas Red conjugated and
>goat anti-mouse fluorescein conjugated). but If secondary antibodies
>cross-react, what should I do? My friend told me that I could use
>depletion method. but I am not sure.
>Can anybody do me a favor?
>Do You Yahoo!?
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