[Histonet] Cleaved Caspase-3 antibody and thanks

Henry, Charlene Charlene.Henry <@t> STJUDE.ORG
Fri Mar 18 09:00:30 CST 2005

I could not get the Cleaved Caspase 3 from Cell Signaling to work for
me; however I ordered it from Sigma Cat. # C5737 and it works great.
Hope this helps.
Also we use a lot of research antibodies and I have found that the Cell
Signaling antibodies do not work very well and they are very expensive.
I was wondering what experience others have had with antibodies from
Cell Signaling.

Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
fax 901-495-3100

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Thursday, March 17, 2005 2:56 PM
To: 'HISTONET' (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Cleaved Caspase-3 antibody and thanks


Is the Cell Signaling cleaved caspase-3 antibody still the best for IHC
on FFPE sections? My in-house antibody bit the dust.

Also, thanks to those who replied about my cell block question...I
passed the info along.


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly <@t> merck.com

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