[Histonet] immunos on whole rat corneas

[Elizabeth Chlipala]

Hello everyone
 
Has anyone had any experience in immunostaining whole rat corneas?  My
biggest concern is permeabilizing the cornea to get sufficient
penetration.  In the past I have used 0.1% Triton X-100 for 50 micron
free floating vibratome sections and that seemed to work quite well for
that application.  Any suggestions would be appreciated.  Thanks in
advance.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
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