[Histonet] uneven sections on slides

[Shirley Powell]

In the past when I had this problem, the mounting media was too thick and
another time I had gotten a bad batch of coverslips, both of which were
easily corrected.  Coverslips have better QC now so I doubt if that is the
problem.  

Shirley

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Julie.Sanders <@t> med.va.gov
Sent: Thursday, March 17, 2005 11:11 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] uneven sections on slides

Hello All,
In the past few days one our pathologists has been complaining about "uneven
sections."  In other words, he's having to focus up and down a lot because
the sections aren't flat on the slide.  Any body have any explanations of
why this could be happening and any solutions?  We haven't been doing
anything different, use plus charged slides and paraplast x-tra paraffin. 15
minutes in a 55 degree C oven and automatic stainer.
Any comments are greatly appreciated.
Julie
Julie Sanders, BA,HT(ASCP)
Supervisor, Anatomic Pathology
VA Medical Center
Cincinnati, Oh. 45220

Julie A. Sanders, BA,HT(ASCP)
Supervisor, Anatomic Pathology
Cincinnati VAMC


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