[Histonet] immunocytochemistry and IHC

[Dana Settembre]

Laura,
I highly recommend that you contact DakoCytomation.
They helped our lab with many aspects of immunohistochemistry.
I often have people tell me that my immunos are one of the best
they've
seen and I have to hand if to Dako for all their help and great
antibodies.
Their antibodies work really nicely and incubation times can be as
short as 15 minutes.
Most of mine are.  Call them and ask them who your representative is
1-800-235-5743.  They have a great customer service team and their
technical service team is so user friendly.  I always had my Rolodex
open
to their number so I could ask them anything.
I have also been to a small basic immunohistochemistry seminar in 
my area that was set up by Dako.
Your lab could benefit from something like that too, if they offer it
in your area.
You can use their "ready to use" antibodies or use their concentrated
ones.
You can do them by hand or get automated. Dako could help you with
either.
Good Luck
Dana Settembre, HT  1-973-972-5232
University Hospital, UMDNJ
Newark, NJ

>>> Laura Fidgen <lfidgen <@t> vt.edu> 3/16/2005 2:19:19 PM >>>
Good afternoon!
We are a small lab trying to begin an IHC and immunocytochemistry 
program.  Our pathologists have given us a list of various antibodies
they 
would like to have and my question is, what is the best way to 
begin?  There are various leukocyte markers, intermediate filaments, 
cytokeratins, hormones, immunoglobulins, and an "other" category
proposed 
and I could use help getting started and pointed in the right 
direction.  Almost all the MT's and HT's here have never been exposed
to 
immunos.  Any help would be GREATLY appreciated.


Laura L. Fidgen, MScF, BSc, HT(ASCP)
Laboratory and Research Practioner
VMRCVM, Histopatholgy/Clinical Pathology Dept.
Blacksburg, VA 24060-0443 


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