[Histonet] I use the Fisher Microprobe Staining system

[Patti Loykasek]

We use the Microprobe & love it. It's inexpensive, uses little reagent, very
consistent heat & results. It does have a few quirks that you need to watch
out for - be sure & use cap-gap slides, put tissue low on slides, carefully
watch that your reagents drain & pick up well. It's important to have nice,
thin sections. Once you get the feel of what to watch for, it's great. Be
sure there are surfactants in your buffer. If you need more info, contact
me. Good luck.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA



> 
>  I love the Fisher Microprobe System, it is simple and easy for a small
>  lab  to  get good consistent staining. It's reasonably priced too, and
>  you save money in reagents. I  hope this helps- Good Luck!
> 
>  Christine Tambasco HT (ASCP)
> 
>  St. Mary's Hospital
> 
>  Amsterdam, New York 12070
>  ph-518-841-7287
>> From: Kelly D Mcqueeney <kelly.mcqueeney <@t> bms.com>
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Anyone use the Fisher Microprobe Staining system?
>> Date: Mon, 14 Mar 2005 14:06:29 -0500
>> 
>> Hi Histonetters,
>> Has anyone used the Fisher Microprobe Staining system? Do you have
>> any problems? We are interested in a very simple staining system for
>> only one 60-minute incubation in hot ligand. I have used the Thermo
>> Sequenza but we are having technical issues, plus loading the slides
>> is a pain. We do not work with more than 20 slides at a time (and
>> using radioligand, clean-up is an issue). I would appreciate no
>> response from reps concerning complex, automated systems, we have
>> already determined they are not right for us.
>> 
>> Thanks,
>> Kelly
>> 
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