[Histonet] bile pigment

Joanne Mauger MAUGER <@t> email.chop.edu
Fri Mar 11 13:26:59 CST 2005

Hi Everyone,
Does anyone know of a way to remove bile pigment from tissue sections?
I have been searching, and can't find any methods.

>>> "Sandy Cheasty" <SCheasty <@t> memorialcare.org> 03/11/05 1:43 PM >>>
Thanks to all who gave me suggestions... and the winner is:
1.	Chromic acid 10 min room temp (any brand)
2.	Tap H2O, rinse well
3.	Sodium bisulfite 1 min (any brand)
4.	Tap H2O, rinse well
5.	DI water x 3 changes
6.	SIGMA meth silver reagent (1 vial makes 100 ml, that will last
least a week if you don't add the Borax. We use 25 ml at a time and add
1 ml
of SIGMA Borax)
      Place slides in microwave proof slide holder, zap in MW at full
in 15 seconds bursts to a maximum of 3 bursts. After each burst pour
reagent back and forth into another acid clean container         to
distribute the heat layers in the solution and check the control for
adequate development.
7.	Tap H2O, rinse well
8.	DI water x 2-3 changes
9.	Gold chloride
10.	yadda
11.	yadda
12.	yadda
13.	Coverslip
The fungal and pneumocytis  organism staining is thorough, delicate,
and without background. Just like mother to used make. I tried this
Rowley reagents and Polyscientific reagents, the pneumocystis didn't
well and the background was butt-ugly.

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