[Histonet] F4/80 on human tumour xenografts in mouse

[ncragg]

Hello,

Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown 
in nude mice to look for infiltrating macrophages?  We are using the Caltag 
rat monoclonal, BM8, although we've got it working nicely on normal mouse 
spleen, the staining pattern is unusual in the tumour xenografts, staining 
is seen around the human tumour cells (almost like tumour matrix) rather 
than infiltrating mouse cells.  Has anyone seen this pattern before or can 
suggest what is happening here?

Thank you in advance,

Nicola

Nicola Cragg BSc
Epistem Ltd
Manchester, UK