[Histonet] LCA antibody for B5 tissue

[Moklebust, Amanda C]

Patti,

	I have been pretreating the slides with Dako's Target Retrival
Solution pH 6.1 in a steamer for 20 minutes.  The slides do not get treated
to remove mercury pigment.  I've gotten two replies recommending a different
clone (2B11/PD7/26) from Dako.  I don't think they were posted to everyone.
Thanks for the help. 

Amanda 


-----Original Message-----
From: Patti Loykasek [mailto:ploykasek <@t> phenopath.com]
Sent: Wednesday, March 09, 2005 12:29 PM
To: histonet
Subject: Re: [Histonet] LCA antibody for B5 tissue


Amanda,
    I¹m almost positive I¹ve had this antibody work on B5 fixed tissues,
mainly bone marrows. What type of antigen retrieval are you using? This
could make a difference. Are you doing any type of treatment to remove the
precipitated mercuric chloride? This can lead to false negatives with some
antibodies.
I¹ve routinely used a citrate based heat pretreatment. This recovers the
antigen nicely and removes the mercuric chloride(in most cases).
Hope this helps. 

Patti Loykasek
PhenoPath Laboratories
Seattle, WA> 


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