[Histonet] LCA antibody for B5 tissue

[Moklebust, Amanda C]

Dear Histonetters, 

	Do any of you know of a clone for CD45-LCA that stains B5 fixed
tissue?  We are using a clone from DakoCytomation, anti-CD45, T29/33.  It
stains formalin fixed tissue, but I see no staining in the B5 fixed tonsil
control.  I have tried different blocks of tonsil with the same result.  All
of the blocks stain with CD3 and CD20, so my guess is that this antibody
does not recognize the antigen after B-5 fixation.

	Please let me know if you have had any success with CD45-LCA in B5
fixed tissue.  Replies telling me not to use B5 are not helpful. I know it
has a lot of negative qualities, and I would love to avoid the whole issue.
Unfortunately, I have to work with an archive of tissue fixed in B5. 

Thanks for your assistance,

Amanda Moklebust, HTL(QIHC)
Seattle Cancer Care Alliance



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