[Histonet] technovit 9000 new

[Gayle Callis]

Technovit 9000 is a methyl methacrylate kit.  To remove MMA from thin 
microtomed sections, soak  sections in 60C xylene X 3 changes, proceed with 
rehydration.  You probably will have to use Neil Hands rather stringent 
pressure cooker retrievals for IHC.   He has a publication in J of 
Histotechnology a few years back on how he succeeded with retrieval using 
MMA.  You can access the journal for first page/abstract of publication via 
www.NSH.org   click on J of Histotechnology.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)