[Histonet] immunofluorescence staining in frozen tissue

Kelly Yang yang5yc <@t> yahoo.com
Mon Mar 7 19:01:14 CST 2005

Dear histonetters,


We are working on immunofluorescence staining with two markers, ki67 and DNMT1, in human bladder tissue. I had tried ki67 from Dako and DNMT1 from Imgenex, but none of them give us the good result. 


Does anyone have a protocol for staining frozen tissue with Ki67 or DNMT1? Also which antibody are you recommending? 

Thank you for your help in advance. 


Kelly Yang
Graduate student 
Department of Epidemiology
School of Public Heath
University of California, Los Angeles
ext. 57795
yangyc <@t> ucla.edu
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