[Histonet] mouse heart morphology

[Stephen Peters M.D.]

David,
 
Here is a idea based on no experience:
 
Inject the fresh heart with OCT.  Place a landmark with ink or suture to maintain orientation.
Freeze it whole. Do either A or B
 
A) Embed the entire heart in the desired orientation and simply trim down until you reach the desired plane of section.
 
B) Let the frozen heart warm to about -15 C. Now with a scalpel, cut it in the desired plane and embed the halves to achieve the desired plane of section.
 
As far as getting high quality frozens section slides that is quite possible with good technique.
 
Stephen


Stephen Peters M.D. 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 
www.pathologyinnovations.com 

Senior Attending Pathologist 
Hackensack University Medical Center 
201 996 4836