[Histonet] RE: Histonet Digest, Vol 16, Issue 7
Ray Sukumar
rsukumar <@t> dpspa.com
Fri Mar 4 04:55:33 CST 2005
How do we advertize for a Histology Manager and a histotech at your website?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, March 03, 2005 11:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 16, Issue 7
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Today's Topics:
1. RE: Need an opinion[Scanned] (Kemlo Rogerson)
2. Training (Fawn Jones)
3. RE: block alignment (Dawson, Glen)
4. RE: block alignment (Weems, Joyce)
5. Basic ISH and IHC (Julie.Sanders <@t> med.va.gov)
6. RE: block alignment (Bartlett, Jeanine)
7. CAP survey slids (ktournear <@t> cox.net)
8. RE: block alignment (Weems, Joyce)
9. Shandon Hypercenter XP (Snider, Deanna)
10. RE: block alignment (Bartlett, Jeanine)
11. Re: Basic ISH and IHC (Kelly D Mcqueeney)
12. Re: TH and golgi-cox? (Geoff McAuliffe)
13. RE: block alignment (Weems, Joyce)
14. RE: block alignment (Bartlett, Jeanine)
15. RE: Need an opinion (Charles.Embrey)
16. block alignment (amosbrooks <@t> earthlink.net)
17. UK prices (Edwards, R.E.)
18. RE: block alignment (Bartlett, Jeanine)
19. RE: block alignment (Bonnie Whitaker)
----------------------------------------------------------------------
Message: 1
Date: Thu, 3 Mar 2005 12:36:57 -0000
From: Kemlo Rogerson <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] Need an opinion[Scanned]
To: "'Heather.A.Harper <@t> pcola.med.navy.mil'"
<Heather.A.Harper <@t> pcola.med.navy.mil>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1030B679AD69D6119C3F00080210DD9D05A3F04C <@t> bhrv-nt-11.bhrv.nwest.nhs.uk>
Content-Type: text/plain
You need at the very least a Standard Operational procedure for 'grossing'.
This will have each specimen and how the Pathologist has trained you to 'cut
it'. For example skin biopsies of a ?mole, would be cut TS with three slices
across the mole. The ends retained 'just in case'. The same for anything
else you dissect or 'put in'. That is each sample with have a procedure for
you to follow, which you do with no alteration.
I would have thought that if a Pathologist sat in for a few days whilst you
carried out the 'cut up' then you would learn quickly; if I was the
Pathologist then I would occasionally revisit to make sure. It really
depends on your experience and training; the issues relating to tissue
'cross over' (on forceps, cut up board, etc) are maybe the most important,
but other issues concerning under fixed tissue and your ability to know when
to ask for help given an unusually finding need to be addressed. The latter
is one of the most important; you can train people to do almost anything,
the trick is to get them to recognise when they are 'stepping out' of that
training (i.e. it's not a mole, but the Clinical data and gross macro
suggests that a melanoma may be a likelihood, then you refer).
Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194
-----Original Message-----
From: Heather.A.Harper <@t> pcola.med.navy.mil
[mailto:Heather.A.Harper <@t> pcola.med.navy.mil]
Sent: 03 March 2005 11:54
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need an opinion[Scanned]
I had written last week in regards to the sudden work change of me having
to learn how to gross. I'm going to go ahead and learn but I need an
opinion. I have 30 days to learn how to gross. Presently, the pathologist is
training a cyto tech to gross. She got trained last week and this week is
flying solo and she is very unsure of what she is doing and I have been
telling her what she has to do. She already lost 2 cxbx on a case. I also
observed this same type of training with my military co-worker. Trained one
week, flying solo the next and that particular pathologist isn't always in
his office. So she would have to de-glove and pick up the phone and call a
pathologist. If you were in my situation, tell me in your opinion what 30
days of training would entail because maybe I'm expecting too much. I
believe for those 30 days, the pathologist should be standing in the
background, watching, listening and observing, no matter how fast you caught
on to grossing and be there for any questions. I start training April 1st
and would just like to know what the histo world would expect. Thanks in
advance for any opinions.
Heather A. Harper
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Thu, 3 Mar 2005 08:20:38 -0500
From: "Fawn Jones" <fjones <@t> namsa.com>
Subject: [Histonet] Training
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<915E55B02E236E4D95258B181EEF6317075DC5 <@t> namsams01.namsa.int>
Content-Type: text/plain; charset="us-ascii"
I am having a new situation starting to occur, where our company wants
to hire a high school student to help ship our slides and now they are
telling us she can gross our tissues also. We work in a medical device
testing company where we gross everything from muscles, to spines, to
all organs from mice, rats, rabbits, dogs, swine, sheep and guinea pigs.
These are not necessarily easy things to gross as we have to look for
test articles and implant sites (which may not be visible
macroscopically), and determine if there are any changes in the tissues.
I'm not sure we are qualified enough ourselves to be doing these things
let alone a high school senior. If anybody could send any information
on the qualifications needed for this type of work so that we could show
our managers, (which are not histotechs and have no histology
experience), that hiring a high school senior to do grossing is not a
good idea. We also will not have a pathologist on hand to help with
grossing of these tissues or for training. There are no set standards
for grossing as most of our special studies have different test articles
and different things they are looking for, so no two of these studies
are really ever the same.
Thank you in advance
Fawn Jones
------------------------------
Message: 3
Date: Thu, 3 Mar 2005 07:27:43 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] block alignment
To: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<D2401DE71F59D71184BC00D0B7479B91C4BB9C <@t> milw_mail1.milw.dynacare.com>
Content-Type: text/plain; charset="iso-8859-1"
Jeanine,
I believe we have 7 Histo Collimators here and I've used one for at least 5
or 6 years and I couldn't go without it. Using the Collimator, I usually
waste less than 10 microns worth of tissue on an outside block face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block alignment products
such as the Microtome Aligner from Newcomer or the Histo Collimator? We
receive a huge number of outside blocks and I adjust my block holder
accordingly, but others in the group say it's easier to re-embed all
outside blocks (Yikes!) and then have every microtome aligned the same.
I was just wondering if these aligners work and if so perhaps it could
be an alternative for those individuals that normally would rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 3 Mar 2005 08:44:18 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] block alignment
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>, "Bartlett,
Jeanine" <JQB7 <@t> CDC.GOV>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7EA9D1DD <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
>From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and I've used one for at least 5
or 6 years and I couldn't go without it. Using the Collimator, I usually
waste less than 10 microns worth of tissue on an outside block face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block alignment products
such as the Microtome Aligner from Newcomer or the Histo Collimator? We
receive a huge number of outside blocks and I adjust my block holder
accordingly, but others in the group say it's easier to re-embed all
outside blocks (Yikes!) and then have every microtome aligned the same.
I was just wondering if these aligners work and if so perhaps it could
be an alternative for those individuals that normally would rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Thank you. Saint Josephs Health System, Inc.
------------------------------
Message: 5
Date: Thu, 3 Mar 2005 07:46:23 -0600
From: Julie.Sanders <@t> med.va.gov
Subject: [Histonet] Basic ISH and IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<457381D92B01BD44B21CF37CC02EBDFD02927596 <@t> vhacinexc5.v10.med.va.gov>
Content-Type: text/plain; charset="iso-8859-1"
Hello all,
I am looking for any books/information regarding basic (very basic) theories
and explanations for ISH and IHC. We do ISH in our lab and know the
technique, but not the theory, so I'm trying to find information on these
subjects. I've looked at Amazon.com, but most of the books seem way beyond
"basic." What we are looking for is something that can give us at least
"beginners" information. I spoke to our Ventana rep. and suggested that
Ventana should put on regional workshops. (are you reading this Ventana?)
With so many histology labs using this technology it would be helpful to
have background. Its easy to slap some slides on a machine, put the
dispensers on, click start and walk away....now we need to know the how and
why we do this. Any information/suggestions would be greatly appreciated!
Julie Sanders, BA, HT(ASCP)
Supervisor, Anatomic Pathology
VA Medical Center
3200 Vine St. ML113
Cincinnati, Ohio 45220
------------------------------
Message: 6
Date: Thu, 3 Mar 2005 08:48:54 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] block alignment
To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C07615D7F <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Richard-Allan
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 8:44 AM
To: Dawson, Glen; Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and I've used one for
at least 5
or 6 years and I couldn't go without it. Using the Collimator,
I usually
waste less than 10 microns worth of tissue on an outside block
face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block alignment
products
such as the Microtome Aligner from Newcomer or the Histo
Collimator? We
receive a huge number of outside blocks and I adjust my block
holder
accordingly, but others in the group say it's easier to re-embed
all
outside blocks (Yikes!) and then have every microtome aligned
the same.
I was just wondering if these aligners work and if so perhaps it
could
be an alternative for those individuals that normally would
rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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message to the intended recipient, you are hereby notified that any
disclosure, copying, distribution or the taking of any action in
reliance on the contents of this information is strictly prohibited. If
you have received this communication in error, please notify us
immediately by replying to the message and deleting it from your
computer.
Thank you. Saint Joseph's Health System, Inc.
------------------------------
Message: 7
Date: Thu, 3 Mar 2005 8:59:31 -0500
From: <ktournear <@t> cox.net>
Subject: [Histonet] CAP survey slids
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20050303135931.HMQC19936.fed1rmmtao09.cox.net <@t> smtp.west.cox.net>
Content-Type: text/plain; charset=ISO-8859-1
Hi out there,
I have the new CAP survey IHC slides for 5 pathologists. My problem is, is
that I only have 4 cases/10 slides each case and each pathologist is
supposed to do this now. Can anyone tell me how this is done? I don't think
I have enough slides for all of them.
Thanks!!
Kim Tournear, HT (ASCP) QIHC
Specialists in Dermatology PLLC
Tucson, AZ
------------------------------
Message: 8
Date: Thu, 3 Mar 2005 09:10:41 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] block alignment
To: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7EA9D1DF <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
It only fits their microtomes.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 8:48 AM
To: Weems, Joyce; Dawson, Glen; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Richard-Allan
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 8:44 AM
To: Dawson, Glen; Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and I've used one for
at least 5
or 6 years and I couldn't go without it. Using the Collimator,
I usually
waste less than 10 microns worth of tissue on an outside block
face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block alignment
products
such as the Microtome Aligner from Newcomer or the Histo
Collimator? We
receive a huge number of outside blocks and I adjust my block
holder
accordingly, but others in the group say it's easier to re-embed
all
outside blocks (Yikes!) and then have every microtome aligned
the same.
I was just wondering if these aligners work and if so perhaps it
could
be an alternative for those individuals that normally would
rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_____
Confidentiality Notice ** The information contained in this
message may be privileged and is confidential information intended for
the use of the addressee listed above. If you are neither the intended
recipient nor the employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
disclosure, copying, distribution or the taking of any action in
reliance on the contents of this information is strictly prohibited. If
you have received this communication in error, please notify us
immediately by replying to the message and deleting it from your
computer.
Thank you. Saint Joseph's Health System, Inc.
Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the
addressee listed above. If you are neither the intended recipient nor the
employee or agent responsible for delivering this message to the intended
recipient, you are hereby notified that any disclosure, copying,
distribution or the taking of any action in reliance on the contents of this
information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer.
Thank you. Saint Josephs Health System, Inc.
------------------------------
Message: 9
Date: Thu, 3 Mar 2005 09:19:23 -0500
From: "Snider, Deanna" <dsnider <@t> shrinenet.org>
Subject: [Histonet] Shandon Hypercenter XP
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A505C26A8103D511B3CE00306E06479102AB234D <@t> CINFS4>
Content-Type: text/plain; charset="iso-8859-1"
Shriners Hospital for Children
Research Dept.
Cincinnatti, Oh
513-872-6388
Hello again everyone in Histoland!
Does anyone use this processor? I had not used it prior to accepting this
position. What seems to be the problem is that it seems to have a lot of
carry over from station to station. I have read the manual, did all the
maintenance it suggests, and I diligently keep up the daily routine
maintenance. I process very small samples of clinically engineered skin.
The largest workload I have ever had here was 34 blocks. Usually it is more
like 8- 10 cassettes.
I just changed all the reagents yesterday, including both paraffins. I had
a run with 4 cassettes overnight and this morning the first paraffin station
has a very strong xylene smell. Has anyone else found this to be a problem
with this particular piece of equipment? I don't recall having this much of
a problem with this in the VIP's or MVPs which I have worked with for the
last 15 years. I read through the archives to see if anyone had mentioned
this and found nothing, so your comments will be greatly appreciated.
Thanks
Deanna Snider HT
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Message: 10
Date: Thu, 3 Mar 2005 09:14:47 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] block alignment
To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C07615D80 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
The literature I have shows that it fits the majority of Microms. I
know they are now the distributor but that hasn't always been the case.
The one Newcomer has fits Microms and Leica, I believe.
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 9:11 AM
To: Bartlett, Jeanine; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
It only fits their microtomes.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 8:48 AM
To: Weems, Joyce; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Richard-Allan
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 8:44 AM
To: Dawson, Glen; Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on
behalf of
Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and I've used
one for
at least 5
or 6 years and I couldn't go without it. Using the
Collimator,
I usually
waste less than 10 microns worth of tissue on an outside
block
face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block
alignment
products
such as the Microtome Aligner from Newcomer or the Histo
Collimator? We
receive a huge number of outside blocks and I adjust my
block
holder
accordingly, but others in the group say it's easier to
re-embed
all
outside blocks (Yikes!) and then have every microtome
aligned
the same.
I was just wondering if these aligners work and if so
perhaps it
could
be an alternative for those individuals that normally
would
rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_____
Confidentiality Notice ** The information contained in
this
message may be privileged and is confidential information
intended for
the use of the addressee listed above. If you are neither the
intended
recipient nor the employee or agent responsible for delivering
this
message to the intended recipient, you are hereby notified that
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------------------------------
Message: 11
Date: Thu, 03 Mar 2005 09:18:09 -0500
From: Kelly D Mcqueeney <kelly.mcqueeney <@t> bms.com>
Subject: Re: [Histonet] Basic ISH and IHC
To: Julie.Sanders <@t> med.va.gov
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42271CA1.2020001 <@t> bms.com>
Content-Type: text/plain; charset=us-ascii; format=flowed
Try the Roche website, it's pretty informative
http://us.diagnostics.roche.com/
click on *research*
then *printed materials*
then *non-radioactive In Situ Hybridization
Application Manual. *They give a nice description of the experiment,
reagents, and WHY you use them. *
For IHC, try *Current Protocols in Immunology. *
http://www.does.org/masterli/cpi.html
*
Julie.Sanders <@t> med.va.gov wrote:
>Hello all,
>I am looking for any books/information regarding basic (very basic)
theories
>and explanations for ISH and IHC. We do ISH in our lab and know the
>technique, but not the theory, so I'm trying to find information on these
>subjects. I've looked at Amazon.com, but most of the books seem way beyond
>"basic." What we are looking for is something that can give us at least
>"beginners" information. I spoke to our Ventana rep. and suggested that
>Ventana should put on regional workshops. (are you reading this Ventana?)
>With so many histology labs using this technology it would be helpful to
>have background. Its easy to slap some slides on a machine, put the
>dispensers on, click start and walk away....now we need to know the how and
>why we do this. Any information/suggestions would be greatly appreciated!
>
>Julie Sanders, BA, HT(ASCP)
>Supervisor, Anatomic Pathology
>VA Medical Center
>3200 Vine St. ML113
>Cincinnati, Ohio 45220
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 12
Date: Thu, 03 Mar 2005 09:43:29 -0800
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] TH and golgi-cox?
To: ninian H <ninian_h <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42274CC1.7090405 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Hi Ninian:
As for Golgi-Cox, John Kiernan is the man to ask. As for a nuclear
stain to go with TH, I have used Toluidine Blue, Thionin, Cresyl Violet
and Celestine Blue B with excellent results. Assuming you are
demonstrating TH with DAB, get some of Vector's "Intense", it turns the
DAB a nice golden color which goes very well with any blue nuclear
stain. If you are intensifying the DAB with nickel+cobalt a red nuclear
stain would look nice. I don't know why neutral red did not work for
you, I suggest a fresh batch. Or try "Scarba Red", a neutral red
variant. See Slidders et al., J. Pathol. Bacterol. 75:476-478, 1958.
Geoff
ninian H wrote:
>Hi all,
>Does anyone know about combining golgi-cox with tyrosine hydroxylase
staining? the main purpose of mine is to distinguish different layers of
cortex and TH positive fibers. I'll appreciate any information that might
help me to see both pyramydal cells and TH containing fibers in cortical
areas.
>Also co-staining with TH and neutral red didn't work out, and TH -stained
neurons didn't stain with red color , any idea or suggestion?
>Thank you in advance,
>Ninian, ph.D student
>
>
>histonet-request <@t> lists.utsouthwestern.edu wrote:
>Send Histonet mailing list submissions to
>histonet <@t> lists.utsouthwesten.edu
>
>To subscribe or unsubscribe via the World Wide Web, visit
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>or, via email, send a message with subject or body 'help' to
>histonet-request <@t> lists.utsouthwestern.edu
>
>You can reach the person managing the list at
>histonet-owner <@t> lists.utsouthwestern.edu
>
>When replying, please edit your Subject line so it is more specific
>than "Re: Contents of Histonet digest..."
>
>
>Today's Topics:
>
>1. RE: removal of carbohydrates/glycosylation
>(Edmondson David (RBV) NHS Christie Tr)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Wed, 2 Mar 2005 17:48:49 -0000
>From: "Edmondson David \(RBV\) NHS Christie Tr"
>
>Subject: RE: [Histonet] removal of carbohydrates/glycosylation
>To: "Sharon Cooperman"
>Cc: "Histonet \(E-mail 2\)"
>Message-ID:
>
>Content-Type: text/plain; charset="iso-8859-1"
>
>
>I wonder that oxidation will do this but that there will be consequences
for the antigens that you are working with. Years ago, I remember using
Periodic acid and then Borohydride as a peroxidase block but that PD726
samples from Oxford did not work at all. The LCA is obviously a run of the
mill antibody now and carbohydrate moities a significant part thereof.
>Any suggestions/
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sharon
>Cooperman
>Sent: 02 March 2005 15:02
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] removal of carbohydrates/glycosylation
>
>
>Hi,
>
>Does anyone know of a method to remove carbohydrate
>modifications/glycosylation from proteins in tissue sections on
>slides prior to immunohistochemistry? (I know it's a weird
>question.) Any suggestions, references, etc. would be greatly
>appreciated.
>
>Thanks,
>Sharon
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 13
Date: Thu, 3 Mar 2005 09:39:44 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] block alignment
To: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7EA9D1E0 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Unless it's changed, it doesn't fit Leicas.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 9:14 AM
To: Weems, Joyce; Dawson, Glen; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
The literature I have shows that it fits the majority of Microms. I
know they are now the distributor but that hasn't always been the case.
The one Newcomer has fits Microms and Leica, I believe.
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 9:11 AM
To: Bartlett, Jeanine; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
It only fits their microtomes.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 8:48 AM
To: Weems, Joyce; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Richard-Allan
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 8:44 AM
To: Dawson, Glen; Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on
behalf of
Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and I've used
one for
at least 5
or 6 years and I couldn't go without it. Using the
Collimator,
I usually
waste less than 10 microns worth of tissue on an outside
block
face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with block
alignment
products
such as the Microtome Aligner from Newcomer or the Histo
Collimator? We
receive a huge number of outside blocks and I adjust my
block
holder
accordingly, but others in the group say it's easier to
re-embed
all
outside blocks (Yikes!) and then have every microtome
aligned
the same.
I was just wondering if these aligners work and if so
perhaps it
could
be an alternative for those individuals that normally
would
rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Confidentiality Notice ** The information contained in this message may be
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------------------------------
Message: 14
Date: Thu, 3 Mar 2005 09:43:40 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] block alignment
To: "Weems, Joyce" <JWEEMS <@t> sjha.org>, "Dawson, Glen"
<GDawson <@t> dynacaremilwaukee.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C07615D81 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
The Histo Collimator doesn't, but the one from Newcomer does.
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 9:40 AM
To: Bartlett, Jeanine; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Unless it's changed, it doesn't fit Leicas.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 9:14 AM
To: Weems, Joyce; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
The literature I have shows that it fits the majority of
Microms. I
know they are now the distributor but that hasn't always been
the case.
The one Newcomer has fits Microms and Leica, I believe.
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 9:11 AM
To: Bartlett, Jeanine; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
It only fits their microtomes.
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Thu 3/3/2005 8:48 AM
To: Weems, Joyce; Dawson, Glen;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Richard-Allan
-----Original Message-----
From: Weems, Joyce [mailto:JWEEMS <@t> sjha.org]
Sent: Thursday, March 03, 2005 8:44 AM
To: Dawson, Glen; Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
From which company?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
on
behalf of
Dawson, Glen
Sent: Thu 3/3/2005 8:27 AM
To: Bartlett, Jeanine;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] block alignment
Jeanine,
I believe we have 7 Histo Collimators here and
I've used
one for
at least 5
or 6 years and I couldn't go without it. Using
the
Collimator,
I usually
waste less than 10 microns worth of tissue on an
outside
block
face-in.
Thumbs Way Way Up on block aligners,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
Sent: Wednesday, March 02, 2005 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Hi:
Does anyone out there have any experience with
block
alignment
products
such as the Microtome Aligner from Newcomer or
the Histo
Collimator? We
receive a huge number of outside blocks and I
adjust my
block
holder
accordingly, but others in the group say it's
easier to
re-embed
all
outside blocks (Yikes!) and then have every
microtome
aligned
the same.
I was just wondering if these aligners work and
if so
perhaps it
could
be an alternative for those individuals that
normally
would
rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_____
Confidentiality Notice ** The information
contained in
this
message may be privileged and is confidential
information
intended for
the use of the addressee listed above. If you are
neither the
intended
recipient nor the employee or agent responsible for
delivering
this
message to the intended recipient, you are hereby
notified that
any
disclosure, copying, distribution or the taking of any
action in
reliance on the contents of this information is strictly
prohibited. If
you have received this communication in error, please
notify us
immediately by replying to the message and deleting it
from your
computer.
Thank you. Saint Joseph's Health System, Inc.
_____
Confidentiality Notice ** The information contained in
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message may be privileged and is confidential information
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the use of the addressee listed above. If you are neither the
intended
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you have received this communication in error, please notify us
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Confidentiality Notice ** The information contained in this
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recipient nor the employee or agent responsible for delivering this
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------------------------------
Message: 15
Date: Thu, 3 Mar 2005 08:51:08 -0600
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] Need an opinion
To: <Heather.A.Harper <@t> pcola.med.navy.mil>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C320919A1C432845938BCD8CC3294A30205DF2 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
Just for a comparison..... Pathologists' Assistants study two years to
complete their degree to be able to gross. One week hardly seems
adequate. Your Doc's must be glad that it is near to impossible to sue
military doctors for malpractice. Poor patients.
Charles Embrey
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Heather.A.Harper <@t> pcola.med.navy.mil
Sent: Thursday, March 03, 2005 5:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need an opinion
I had written last week in regards to the sudden work change of me
having
to learn how to gross. I'm going to go ahead and learn but I need an
opinion. I have 30 days to learn how to gross. Presently, the
pathologist is
training a cyto tech to gross. She got trained last week and this week
is
flying solo and she is very unsure of what she is doing and I have been
telling her what she has to do. She already lost 2 cxbx on a case. I
also
observed this same type of training with my military co-worker. Trained
one
week, flying solo the next and that particular pathologist isn't always
in
his office. So she would have to de-glove and pick up the phone and call
a
pathologist. If you were in my situation, tell me in your opinion what
30
days of training would entail because maybe I'm expecting too much. I
believe for those 30 days, the pathologist should be standing in the
background, watching, listening and observing, no matter how fast you
caught
on to grossing and be there for any questions. I start training April
1st
and would just like to know what the histo world would expect. Thanks in
advance for any opinions.
Heather A. Harper
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Thu, 3 Mar 2005 10:33:39 -0500 (GMT-05:00)
From: amosbrooks <@t> earthlink.net
Subject: [Histonet] block alignment
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<29712159.1109864020294.JavaMail.root <@t> statler.psp.pas.earthlink.net>
Content-Type: text/plain; charset=us-ascii
Jeanine,
I like to think about it as if I were the patient. If I had a focal
lesion and it was on the surface of the block and some one decided that it
would be easier to re-embed and re-face the block than to align the
microtome and the lesion was faced off, I'd be really upset. Re-embedding
the blocks is a rather dumb idea because you need to cut into the block to
get a full face anyway, there is bound to be tissue loss. Aligning the block
is a really quick process that can be done easily once you are familiar with
doing it. You just need to know what to do. Know the tools you are working
with and life will be easier for everyone. Re-embedding all the blocks is
going to take a lot of time (you certainly aren't saving time with that
option) and you are introducing another potential source of problems. What
if the cassettes accidentally got switched or tissue from one block got
mixed in with tissue from another. It can easily happen when re-embedding a
LOT of blocks. It's just not worth it, re-align don't re-embed unless it's
absolutly necessary.
Just my $0.02
Amos
------------------------------
Message: 3
Date: Wed, 2 Mar 2005 13:42:22 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: [Histonet] block alignment
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C050B55B2 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Hi:
Does anyone out there have any experience with block alignment products
such as the Microtome Aligner from Newcomer or the Histo Collimator? We
receive a huge number of outside blocks and I adjust my block holder
accordingly, but others in the group say it's easier to re-embed all
outside blocks (Yikes!) and then have every microtome aligned the same.
I was just wondering if these aligners work and if so perhaps it could
be an alternative for those individuals that normally would rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
------------------------------
Message: 17
Date: Thu, 3 Mar 2005 15:49:09 -0000
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] UK prices
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F705C75191 <@t> Saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
I need to get some idea of how much it costs to process, cut and
stain a block of tissue, any help/ideas most gratefully received.
Richard Edwards
MRC TOXICOLOGY UNIT
LEICESTER...U.K.......
------------------------------
Message: 18
Date: Thu, 3 Mar 2005 10:54:20 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] block alignment
To: <amosbrooks <@t> earthlink.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C07615D86 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
I appreciate all of the advice. Amos here is on EXACTLY the same page
that I am. But I needed some evidence that I'm on the right track so
when I present my concerns at our staff meeting it won't seem like just
a difference in technique among techs.
Thanks again.
Jeanine
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
amosbrooks <@t> earthlink.net
Sent: Thursday, March 03, 2005 10:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Jeanine,
I like to think about it as if I were the patient. If I had a focal
lesion and it was on the surface of the block and some one decided that
it would be easier to re-embed and re-face the block than to align the
microtome and the lesion was faced off, I'd be really upset.
Re-embedding the blocks is a rather dumb idea because you need to cut
into the block to get a full face anyway, there is bound to be tissue
loss. Aligning the block is a really quick process that can be done
easily once you are familiar with doing it. You just need to know what
to do. Know the tools you are working with and life will be easier for
everyone. Re-embedding all the blocks is going to take a lot of time
(you certainly aren't saving time with that option) and you are
introducing another potential source of problems. What if the cassettes
accidentally got switched or tissue from one block got mixed in with
tissue from another. It can easily happen when re-embedding a LOT of
blocks. It's just not worth it, re-align don't re-embed unless it's
absolutly necessary.
Just my $0.02
Amos
------------------------------
Message: 3
Date: Wed, 2 Mar 2005 13:42:22 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: [Histonet] block alignment
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C050B55B2 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Hi:
Does anyone out there have any experience with block alignment products
such as the Microtome Aligner from Newcomer or the Histo Collimator? We
receive a huge number of outside blocks and I adjust my block holder
accordingly, but others in the group say it's easier to re-embed all
outside blocks (Yikes!) and then have every microtome aligned the same.
I was just wondering if these aligners work and if so perhaps it could
be an alternative for those individuals that normally would rather
re-embed than realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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------------------------------
Message: 19
Date: Thu, 3 Mar 2005 10:41:13 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: RE: [Histonet] block alignment
To: <amosbrooks <@t> earthlink.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000201c5200f$d040e7c0$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain; charset="us-ascii"
I absolutely agree with you!!
Bonnie Whitaker
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
amosbrooks <@t> earthlink.net
Sent: Thursday, March 03, 2005 9:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] block alignment
Jeanine,
I like to think about it as if I were the patient. If I had a focal
lesion and it was on the surface of the block and some one decided that it
would be easier to re-embed and re-face the block than to align the
microtome and the lesion was faced off, I'd be really upset. Re-embedding
the blocks is a rather dumb idea because you need to cut into the block to
get a full face anyway, there is bound to be tissue loss. Aligning the block
is a really quick process that can be done easily once you are familiar with
doing it. You just need to know what to do. Know the tools you are working
with and life will be easier for everyone. Re-embedding all the blocks is
going to take a lot of time (you certainly aren't saving time with that
option) and you are introducing another potential source of problems. What
if the cassettes accidentally got switched or tissue from one block got
mixed in with tissue from another. It can easily happen when re-embedding a
LOT of blocks. It's just not worth it, re-align don't re-embed unless it's
absolutly necessary.
Just my $0.02
Amos
------------------------------
Message: 3
Date: Wed, 2 Mar 2005 13:42:22 -0500
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: [Histonet] block alignment
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C050B55B2 <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
Hi:
Does anyone out there have any experience with block alignment products such
as the Microtome Aligner from Newcomer or the Histo Collimator? We receive
a huge number of outside blocks and I adjust my block holder accordingly,
but others in the group say it's easier to re-embed all outside blocks
(Yikes!) and then have every microtome aligned the same. I was just
wondering if these aligners work and if so perhaps it could be an
alternative for those individuals that normally would rather re-embed than
realign.
Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 16, Issue 7
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