[Histonet] RE: disinfection of cryostat[Scanned]

[Darren James]

Similar thing happened to me with a punch bx of skin.
Frozen section showed lots of granuloma and necrosis. The path asked for a
Wade Fite and it turned out to be leprosy!! AFBs certainly wouldn't die in a
cryostat.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie M
O'Connor
Sent: 03 March 2005 02:57
To: Kemlo Rogerson
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned]

Well, it was a hot day, and I had to get the Popsicles out, anyway . . . . 
.

Scenario:
You get a frozen on a lung sample to rule out tumor - you cut it - you 
know how frozens sometimes shatter and that stuff is all over - it's a 
granuloma - HEY!  That might be Tuberculosis! You're hands have been in 
the cryostat (gloved) and what are the odds you wash your hands between 
cutting a section and staining it?  Now you might have TB bacteria warmed 
up all over everything you touched outside the cryostat.   It could 
happen. 




Kemlo Rogerson <Kemlo.Rogerson <@t> elht.nhs.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/02/2005 07:28 AM

 
        To:     "'Jackie M O'Connor'" <Jackie.O'Connor <@t> abbott.com>
        cc:     histonet <@t> lists.utsouthwestern.edu
        Subject:        RE: [Histonet] RE: disinfection of cryostat[Scanned]


But why did you stick your head in a cryostat?

 

Kemlo Rogerson

Cellular Pathology Manager

East Lancashire Hospitals NHS Trust

DD. 01254-294162

Mobile 0774-9754194

 

-----Original Message-----
From: Jackie M O'Connor [mailto:Jackie.O'Connor <@t> abbott.com] 
Sent: 02 March 2005 12:48
To: Kemlo Rogerson
Cc: 'Charles Scouten'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned]

 


No - but they don't die, either - so when you breathe them into your nice
warm lungs - voila!  They wake up and infect you. 

Jacqueline M. O'Connor HT(ASCP)
Assistant Scientist 
Cancer Research
Jackie.OConnor <@t> abbott.com 




 

Kemlo Rogerson <Kemlo.Rogerson <@t> elht.nhs.uk> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 

03/02/2005 01:50 AM 

 
        To:        "'Charles  Scouten'" <cwscouten <@t> myneurolab.com> 
        cc:        histonet <@t> lists.utsouthwestern.edu 
        Subject:        RE: [Histonet] RE: disinfection of 
cryostat[Scanned]




Do bugs grow at minus 20?

-----Original Message-----
From: Charles Scouten [mailto:cwscouten <@t> myneurolab.com] 
Sent: 01 March 2005 20:04
To: Hopkins, Karen; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: disinfection of cryostat[Scanned]

Why disinfect the cryostat?  Let the durn thing disinfect itself.  IF you
have this requirement, you may want a self disinfecting cryostat.  See the
link:

http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=47510
2&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats
&idsubcategory=182


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hopkins,
Karen
Sent: Tuesday, March 01, 2005 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: disinfection of cryostat

Does anyone have protocol for disinfection of a Reichert Jung cryostat 
that
would meet CAP requirements for disinfection and not involve removing the
microtome from the unit.  We would like to use our Reichert daily but it 
is
too involved to disinfect it weekly (bringing the unit to room temp,
removing the microtome etc...)   I like the Shandons method using
formaldehyde but do not know if I could get away with just wiping the
insides out with formaldehyde or not?   Please offer any advise you think
may help....

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet