[Histonet] removal of carbohydrates/glycosylation

[Edmondson David (RBV) NHS Christie Tr]

I wonder that oxidation will do this but that there will be consequences for the antigens that you are working with.  Years ago, I remember using Periodic acid and then Borohydride as a peroxidase block but that PD726 samples from Oxford did not work at all. The LCA is obviously a run of the mill antibody now and carbohydrate moities a significant part thereof.
Any suggestions/

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sharon
Cooperman
Sent: 02 March 2005 15:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] removal of carbohydrates/glycosylation


Hi,

Does anyone know of a method to remove carbohydrate 
modifications/glycosylation from proteins in tissue sections on 
slides prior to immunohistochemistry?  (I know it's a weird 
question.)  Any suggestions, references, etc. would be greatly 
appreciated.

Thanks,
Sharon
-- 
Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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