[Histonet] antibody anti mouse serum amyloid A (SAA)

[Elizabeth Chlipala]

Nancy

There is not a commercially available antibody to mouse saa.  All the
reference literature talk about a rabbit anti-mouse saa-1, but this
antibody is not available commercially; you have to get it made.  I did
try an antibody to saa from abcam it was a mouse anti-human that was
supposed to work in paraffin sections, but I could not get this to work
in mouse tissue, my negative controls stained as positive as the
antibody.  I stopped working on it after awhile.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dumont,
Nancy
Sent: Tuesday, March 01, 2005 12:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] antibody anti mouse serum amyloid A (SAA)

Hi everybody!
 
I'm a little bit desperate.  Is there anybody here who has used a
commercial antibody against mouse serum amyloid A (SAA) for
immunohistochemistry on mouse sections?  I'm looking for a supplier but
so far, l couldn't find any.  Or if you have a protocol working on
mouse tissue with an anti-human SAA cross reacting with mouse it would
be greatly appreciated.  We tried many protocols but we've got nothing.
Please help me!!!
 
Nancy Dumont
Neurochem Inc.
Laval, Canada
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