[Histonet] Re:Hematoxylin

[Gayle Callis]

Karen,

Contact your Richard Allen sales rep, and have them send smaples of all the 
different types of hematoxylins (Gill formulations, Harris, progressive 
hematoxylins, and Mayers)  or the just ones you want to try.  We  prefer 
hematoxylin 1, a progressive hematoxylin that does not need acid alcohol 
differentiation. It can be shipped to Montana in winter without 
freezing.   Richard Allen has a wonderful hematoxylin and eosin staining 
guide, found on their website to help you out with any of the various 
types.  WE never went wrong with Gill II formulations either.

They also sell eosin y and other reagents needed for hematoxylin staining, 
clarifier (acetic acid solution) and bluing solution.

Good luck

  At 11:01 AM 3/1/2005, you wrote:
>Hello,
>
>I was just wondering what everyone is using for Hematoxylin these days.
>We are one of those rare Histology Labs that makes their own hematoxylin
>and eosin and I just got the OK from our Pathologist to try out some
>commercial brands.  What do you like?  What are you using?  Good and bad
>comments are greatly appreciated.
>
>Thanks!!
>
>Karen Bauer HT(ASCP)
>Histology Supervisor
>Luther Hospital
>Eau Claire, WI
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)