[Histonet] double immunofluorescence in mammary gland tissue
janick.jean-marie
janick.jean-marie <@t> laposte.net
Tue Mar 1 12:30:02 CST 2005
Hi fellows,
I have some problems to make an double immunofluorescence with
paraffin embedded tissue sections.
I suppose, my problem is the protocol of Deparaffinize sections
the bath of xylen and hydratation protocol, who may mask the antigenic determinants
Have you got a protocol for help me ?...
I think to do a treatment of tissue section for unmasked the antigenic determinants ...
Have you got a good protocol for this process?
THANKS !!!
Janick JEAN-MARIE
CRLC INSERM
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