[Histonet] double immunofluorescence in mammary gland tissue

[janick.jean-marie]

Hi fellows,

I have some problems to make an double immunofluorescence with  
paraffin embedded tissue sections.

I suppose, my problem is the protocol of Deparaffinize sections 
the bath of xylen and hydratation protocol, who may mask the antigenic determinants 

Have you got a protocol for help me ?...

I think to do a treatment of tissue section for unmasked the antigenic determinants ...
Have you got a good protocol for this process?

THANKS !!!

Janick JEAN-MARIE
CRLC INSERM 

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