[Histonet] Help

[Edmondson David (RBV) NHS Christie Tr]

Hi,
Did you find an answer, or ewven THE answer.  We went on to use EDTA to make sure of the immuno looking good.  Maybe the 15% Formic gave more "background" or non-specific staining.
Dave
Histology 
Christie Hosp
Manchester UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Traphagan, Patricia
Sent: 22 February 2005 18:05
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help


We are looking for a formic acid decalcifier for our bone marrows. We
have been told that this type of decalcifier works best on bone marrows
when a Kappa and Lambda IP stain is requested. We have tried the BBC
formic acid decalcifier, but found that the length of time it needed to
be in the decal was long (up to 4 fours) and the core was still
calcified. Are there any suggest on what could work?  Thanks

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