[Histonet] re GFP

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Tue Mar 1 02:52:04 CST 2005

I'll concur with john coppens .
I have done std pwax processing and still maintained good signal, provided I 
aq mounted in anti fade mountant. I cut resin sections at 4microns, again 
mounting with antifade mountant and was able to maintain strong enough 
signal to do deconvolution/ z stacks. . Again, I concur with John's 
suggestion to use an anti GFP Ab reagent. I use Molecular Probes'. Some pics 
of FFPWs of fly embryos immunostained for antiGFP are in the gallery on this 
site http://www.immunoportal.com/ 

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