[Histonet] Silly question about deparaffinization

McNaughton, Euan euan.mcnaughtone <@t> agresearch.co.nz
Wed Jun 29 17:43:32 CDT 2005

Wow... Those times all seem huge! We've always just used 2 x 2 min
Xylene, 2 x 1 min 100% EtOH, 1 x 1 min 90% EtOH, 1 x 1 min 70% EtOH then
to water. No problems so long as the solutions are changed when they
start to get dirty.

Euan McNaughton

-----Original Message-----
From: Sebree Linda A. [mailto:la.sebree <@t> hosp.wisc.edu] 
Sent: Thursday, 30 June 2005 2:14 a.m.
To: Eva C Andersson; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Silly question about deparaffinization

We deparaffinize in 3 changes of xylene, 2 changes of 100% ETOH, 1 95%
ETOH and 1 70% ETOH, all for 3 minutes each.  We never skimp on the
xylene times but have been known to shorten the ETOH times if we
manually agitate the slides and are in a real hurry.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva C
Sent: Wednesday, June 29, 2005 8:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Silly question about deparaffinization

Good morning,
I am probably asking a very silly question but I recently spoke to 
someone about deparaffinization and hydration. I found that the times 
that they use and the times that we use are very different. So my 
question to you is this: What lenghts of time do you use for Xylenes, 
100% alcohol, 95% alcohol and 70% alcohol?
It is about deparaffinising charged (+) slides. So nothing fancy. Just
curious. Maybe I can cut back some on the timed that we use which are:
30min Xylenes, 10min 100%, 10min 95% and 6min 70%. Thanks for your
responses. Eva Andersson Georgetown University

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Attention: The information contained in this message and/or attachments
from AgResearch Limited is intended only for the persons or entities
to which it is addressed and may contain confidential and/or privileged
material. Any review, retransmission, dissemination or other use of, or
taking of any action in reliance upon, this information by persons or
entities other than the intended recipients is prohibited by AgResearch
Limited. If you have received this message in error, please notify the
sender immediately.

More information about the Histonet mailing list