[Histonet] MMP-1

[Samantha McMurtrey]

Hello all,

 

I am having a lot of trouble getting any staining for MMP-1.  I am using
Santa Cruz, catalog # sc-21731, lot # A1405. It's a mouse monoclonal
antibody. I have tried dilutions from 1:200 down to 1:25. I incubated the
primary antibody for 30 minutes and the secondary for 30 minutes.  I have
also tried using proteinase K, Target, EDTA, and a pH 9 retrieval. I am
working it up on formalin fixed paraffin embedded tissue. I am mostly using
breast tissue that has atypical ductal hyperplasia (per the pathologist's
request).  I haven't had any luck at all. I am getting no staining. Please
help!

 

Thank you,

 

Samantha McMurtrey, BS, QIHC 

UniPath, LLC

2180 South Leyden Street

Denver, CO 80222

(303) 512-2220