[Histonet] Blue glands

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Thu Jun 23 09:29:57 CDT 2005


My guess would be your hematoxylin.  What kind do you use?  There are some 
commercial brands/types that will stain mucin - check the specs on yours.
There is no reason why the xylene would cause this problem to my 
knowledge.


Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor <@t> abbott.com




"Peterson, Dan" <1dpeterson <@t> meriter.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/23/2005 08:36 AM

 
        To:     <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Blue glands


OK netters,
A question that I'm sure has been covered:
What can we do to get rid of blue color in the glands of our colon bx's,
prostate bx's, etc.
When it happens, it usually seemed to be a clearing problem
(contaminated xylene), but it's only happening on one machine.
All machines get the same recycled xylene. The machine is immaculate. No
clogging of the screen visible.
WIDE open for suggestions.

Daniel R Peterson HT(ASCP)
Histopathology Section Head
General Medical Laboratories
1dpeterson <@t> meriter.com

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