[Histonet] VEGF - question
Andrea T. Hooper
anh2006 <@t> med.cornell.edu
Sun Jun 19 22:16:10 CDT 2005
It is not unexpected that the endothelial cells should stain negative for VEGF.
VEGF normally acts through a paracrine mechanism, whereby endothelial
cells express the receptors for VEGF and the surrounding tissue
(often times tumor cells) are those that secrete VEGF itself. VEGF
can be present in a soluble form or as an ECM bound form so it is
likely that VEGF immunostaining would appear "messy" as you might be
detecting that which is soluble, ECM bound, or even cytoplasmic VEGF
which hasn't yet been secreted from the cell.
The only thing to consider is that many anti-VEGF antibodies are
notorious for being non-specific so plan your experiments with
May I ask the source of your VEGF antibody and if you are staining
human or mouse?
At 5:57 PM +0200 6/19/05, Gudrun Lang wrote:
>Maybe a stupid question. Are endothel cells always stained positiv with
>vascular endothelial growth factor?
>I have got a tissue of a pyogenic granuloma as a positiv control from my
>pathologist. The endothel cells of the vessels are clear negativ, the
>surrounding area is positiv.
>Is this the expected result or is there any mistake?
>Thanks for your help.
More information about the Histonet