[Histonet] RE: Histonet Digest, Vol 19, Issue 25
Rice, Michael
Michael.Rice <@t> holy-cross.com
Fri Jun 17 14:19:36 CDT 2005
I am sure the the Sturkey company who now makes disposables is still sharpening knives
mike rice
holy cross hosp
Ft lauderdale
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Sent: Friday, June 17, 2005 1:07 PM
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Subject: Histonet Digest, Vol 19, Issue 25
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Today's Topics:
1. RE: formic acid decal (DiCarlo, Margaret)
2. RE: xylene substitutes (Shirley Powell)
3. Re: Species specific antibody marker (Jackie M O'Connor)
4. RE: knife sharpening (Due, Brice)
5. RE: Alizarin S mindbender question (Shirley Powell)
6. Histotechnician position available (Spoon, Victoria)
7. Romulin red (Paula Pierce)
8. Re: Romulin red (Jackie M O'Connor)
9. Rodent mast cell IHC (Thomas Crowell)
10. Knife Sharpeneing (HACKERLAB <@t> aol.com)
11. elastic stain (Steven Coakley)
12. Red chromogens (Patti Loykasek)
13. RE: formic acid decal (Elizabeth Chlipala)
14. RE: elastic stain (Patsy Ruegg)
15. RE: Rodent mast cell IHC (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Fri, 17 Jun 2005 09:54:51 -0400
From: "DiCarlo, Margaret" <MDiCarlo <@t> KaleidaHealth.Org>
Subject: RE: [Histonet] formic acid decal
To: 'Trisha Emry' <emry <@t> u.washington.edu>, histo
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<139141F8BAF4A642A945ECC528511AF001F5ECE5 <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Trisha,
I make my own 10% formic acid decal solution and use for large bone samples
without any damage. And I get nice H&E staining.
Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
100 High St.
Buffalo, NY 14203
716-859-1293
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Trisha
Emry
Sent: Thursday, June 16, 2005 17:54
To: histo
Subject: [Histonet] formic acid decal
I need to speed up the decal process on some large bone samples.
What is the highest precentage of formic acid that I can use without
damaging the bones?
I am using sodium formate with formic acid now.
Thanks,
Trisha
Seattle
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------------------------------
Message: 2
Date: Fri, 17 Jun 2005 10:04:51 -0400
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: RE: [Histonet] xylene substitutes
To: histology <@t> gradymem.org, 'Histonet'
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <01LPKB3AZ07K8WXLUG <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii
I have tried Formula 83 from CBG Biotech and it works quite well.
Shirley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histology <@t> gradymem.org
Sent: Friday, June 17, 2005 6:56 AM
To: Histonet
Subject: [Histonet] xylene substitutes
Does anyone currently use xylene substitues in their H&E staining series?
If so, which one have you found to clear best? Did you try several to come
to that conclusion?
We have tried several in the past and are currently using RA ClearRite.
However, all of the sudden our pathologist thinks we can get better
clearing. (I think he has been looking at xylene cleared slides from a
reference lab and remembers how much better xylene
clears.)
If anyone has had wonderful results with any brand of clearing please let us
know.
Thanks,
Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Chickasha, OK
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Message: 3
Date: Fri, 17 Jun 2005 09:08:17 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Species specific antibody marker
To: Colin Nixon <csn1x <@t> udcf.gla.ac.uk>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF136DE5FB.42E78FE8-ON86257023.004D69FA <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Affinity BioReagents, (ABR) has a Mouse MAB (vimentin) which is specific
for Mouse, Human and Rat. (MA3-745). You can use this to distinguish
murine from canine cells, theoretically. I've used a human mitochondria
marker to distinguish human from murine - everything that stains is human
- the ones that don't stain are murine. This should work the same, I
would hope. I haven't found a murine mitochondria marker so far.
Jackie
Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor <@t> abbott.com
"Colin Nixon" <csn1x <@t> udcf.gla.ac.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/17/2005 08:28 AM
Please respond to Colin Nixon
To: <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] Species specific antibody marker
I have received FFPE mouse tissue that has been treated with canine MDCK
cells. It has been established that there are carcinoma cells present in
the mice tissue. What I am trying to establish is whether the tumour has
originated from a canine or murine origin. Does anyone know of any
immunohistochemical markers that would be species specific for this task?
Thanks,
Colin
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------------------------------
Message: 4
Date: Fri, 17 Jun 2005 10:19:43 -0400
From: "Due, Brice" <BDUE <@t> PARTNERS.ORG>
Subject: RE: [Histonet] knife sharpening
To: "Greer, Patricia" <pwg1 <@t> cdc.gov>, "Histonet \(Histonet\)"
<Histonet <@t> pathology.swmed.edu>
Message-ID:
<59C772E2D8EDF345AE1601F5B60B3CB50102705A <@t> PHSXMB7.partners.org>
Content-Type: text/plain; charset="iso-8859-1"
You can also try C.L. Sturkey www.sturkey.com -- they are in PA.
-brice
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Greer,
Patricia
Sent: Friday, June 17, 2005 6:46 AM
To: Histonet (Histonet)
Subject: [Histonet] knife sharpening
Does anyone know of a company that sharpens microtome steel knives? A
colleague here is have a problem with using disposable blades on his
cryostat. The steel knife works well, but now needs sharpening. We
long ago disposed of our knife sharpener and I seem to remember that
there are companies that does microtome knife sharpening.
Thanks,
Pat Greer
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road
Atlanta, GA 30333
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Message: 5
Date: Fri, 17 Jun 2005 10:23:19 -0400
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: RE: [Histonet] Alizarin S mindbender question
To: 'histonet' <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <01LPKBQ7HYL88WXW7U <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=iso-8859-1
Julien,
I have performed the Dawson's technique (page 549 in CFA Culling third
edition) on human embryos years ago using Alizarin to stain bone. I am not
sure if this is suitable for what you are doing but if you contact me I will
be happy to talk to you about what I encountered in the procedure. Your
email address did not show up in the message. My email address is
powell_sa <@t> mercer.edu or you can call me.
Shirley Powell
Mercer University School of Medicine
Macon, GA
478-301-2374
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rittman,
Barry R
Sent: Friday, June 17, 2005 8:46 AM
To: histonet
Subject: RE: [Histonet] Alizarin S mindbender question
Hi Julien
Greetings from Houston where it is never cold. Come visit us sometime if you
want to escape from the north.
I have not worked with fish apart from ingesting several; however I have
used an alizarin/ alcian blue technique several times to stain bone and
cartilage for frogs and rodents.
The technique that you use removes soft tissues and I am unclear as to why
this is necessary. The techniques that I have used have retained the soft
tissue and have made them permeable to clearing agents so that the details
of bone and cartilage are very clear.
My first thought on your technique is that the KOH is made up too strongly
by mistake, can easily happen or that there is still trypsin present and
acting (although the usual pH range for trypsin is 7 to 9). Both would
explain the soft tissue maceration.
I am not sure how long you use the trypsin or its concentration although it
generally is fast acting and it is usually only active for about 3 hours.
Trypsin can be sold with some gelatin in it as a stabilizing agent but not
sure if this is the case here.
The cloudiness may be a reaction between remnants of trypsin and the
alizarin.
Just some thoughts, not sure if these are correct or just BS.
Good luck
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Julien
Lambrey de Souza
Sent: Wednesday, June 08, 2005 3:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Alizarin S mindbender question
Hello histonetters,
In our lab we do routine staining of fish specimens by the Clear and stain
technique of Dingerkus and Uhler 1977, and some of the existing
modifications with very nice results.
But in the last few days, this technique has turned sour....
Something is happening to the fish when they are left in the Alizarin red S
solution made up with 0.5% KOH. The solution has been made fresh several
times but gives the same result each time. The fish seem to contract
(muscular?) so much that the caudal becomes deformed (S shaped), the fin
rays curl up and detach and the cranial elements also seem very fragile. My
first thought was that the KOH solution was too harsh, but then I realized
that the same KOH (0,5%) is used with these fish in the bleaching solution
without harming the fish (apart from normal bleaching).
The protocol is summarized as follows:
1) dehydration in EtOH ---- the fish turn out fine
2) stain in alcian blue solution (EtOH+acetic acid+alcain blue 8GN)---- fish
turn out fine
3) neutralization in borax ---- fish are still fine
4) bleach in H2O2 solution (H2O2 3%+ KOH 0,5%) ---- fish still very nice
5) trypsin digestion (borax+H2O+tryspin) ------ although trypsin has cleared
the specimen of its "meat" the fish is still very nice and fin rays are
intact.
6) Alizarin red S solution (KOH 0,5%+alizarin S to turn solution deep
purple) ----- almost on contact with the solution, the fish rinkles, rays
curl up and detach... the specimen becomes useless. (The solution used to
color the bones red and the specimen was then cleared of excess red by KOH
bath).
I have tried changing borax and KOH solutions with no improvement.
Could it be that Alizarin S has gone bad? Is this even possible? We have had
very high temperatures in the lab lately (30 degrees celsius), could this
have influenced the solutions? I am puzzled. Also, we keep our trypsin in
the -20´C freezer. The freezer has had a problem and freezed to -40´C for a
day. Could this have altered the trypsin in a way that specimen exposition
to this trypsin is incompatible with a later exposition to alizarin? (I am
out of good ideas, so I'm trying absurd ideas....).
If anyone is willing to take a shot, I will accept all suggestions because I
am high and dry.
Thanks for any help.
Julien De souza,
Evolutionary biology,
UQAR, Quebec.
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------------------------------
Message: 6
Date: Fri, 17 Jun 2005 10:25:47 -0400
From: "Spoon, Victoria" <victoria.spoon <@t> bassett.org>
Subject: [Histonet] Histotechnician position available
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <052739589974CC44A7770DA22157C804099B5D <@t> ex3.bassett.org>
Content-Type: text/plain; charset="iso-8859-1"
Bassett Healthcare in Cooperstown, New York has a part-time histotechnician position available immediately with the strong possibility of becoming full time within a year. Responsibilities include full range of histotechniques including embedding, sectioning, routine, special and immuno staining in a hospital based laboratory.
If interested, contact:
Victoria Spoon, Anatomic Pathology Manager
Bassett Hospital
Cooperstown, NY 13326
email: victoria.spoon <@t> bassett.org
(607)547-6357
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------------------------------
Message: 7
Date: Fri, 17 Jun 2005 07:50:54 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] Romulin red
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050617145054.85393.qmail <@t> web50301.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I thought Romulins looked like Vulcans, but were bad, hung out at the neutral zone and had the cloaking device.
Maybe romulin red is like schiff's and is "cloaked" until used. ;)
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
405-759-3953
contact <@t> excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 8
Date: Fri, 17 Jun 2005 09:57:18 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Romulin red
To: Paula Pierce <contact <@t> excaliburpathology.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFC60789F7.3EA8F7EA-ON86257023.0051CA50 <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Biocare is Star Trek oriented - kinda cool and fun - reminds me of doing
business with Ben and Jerry's. (Hey, Hari - send me some ice cream). They
actually have a "Decloaking Chamber" - their pressure cooker for HIER -
as well as a Borg solution for HIER. Check out their website at
www.Biocare.net if you haven't already.
Nice people, too - I might add.
I used the Romulin Red - and it worked just fine. I think, however, that
a Methyl Green counterstain is best with it - kinda Christmas-sy.
Jackie O'
Paula Pierce <contact <@t> excaliburpathology.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/17/2005 09:50 AM
To: histonet <@t> lists.utsouthwestern.edu
cc:
Subject: [Histonet] Romulin red
I thought Romulins looked like Vulcans, but were bad, hung out at the
neutral zone and had the cloaking device.
Maybe romulin red is like schiff's and is "cloaked" until used. ;)
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
405-759-3953
contact <@t> excaliburpathology.com
www.excaliburpathology.com
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------------------------------
Message: 9
Date: Fri, 17 Jun 2005 10:58:02 -0400
From: Thomas Crowell <Thomas.Crowell <@t> biogenidec.com>
Subject: [Histonet] Rodent mast cell IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF5B94B68C.4CFB4C99-ON85257023.0051F619-85257023.005237E4 <@t> biogenidec.com>
Content-Type: text/plain; charset="US-ASCII"
Can anyone tell me if DakoCytomations C-kit CD117 polyclonal antibody
crosses with mouse FFPE mast cells?
Thanks
Tom Crowell
BiogenIdec
Cambridge, MA
------------------------------
Message: 10
Date: Fri, 17 Jun 2005 10:59:14 EDT
From: HACKERLAB <@t> aol.com
Subject: [Histonet] Knife Sharpeneing
To: pwg1 <@t> cdc.gov, Histonet <@t> pathology.swmed.edu
Message-ID: <1ee.3dec0215.2fe43f42 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hi Pat -
I thought I'd pass another option your way - Hacker Instruments located in
Winnsboro, South Carolina sharpens and reconditions knives as well. We also
manufacture/distribute equipment and accessories used in Pathology/Histology -
check out our web site, _www.hackerinstruments.com_
(http://www.hackerinstruments.com/) ; or give us a call; 803) 712-6100.
Be Safe
Shawnelle
Shawnelle Powell
Procurement/Purchasing
Hacker Instruments
803) 712-6100 ext 20
------------------------------
Message: 11
Date: Fri, 17 Jun 2005 08:07:04 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] elastic stain
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050617150704.1180.qmail <@t> web90206.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Good morning everyone,
Is there any elastic stain thats reliable for batch staining, that still give the domonstration of fine/course of a good VVG? Also has anyone the potassium permanganate/oxalic acid prior to the elastic working solution to achieve better staining of elastic fibers? Any opinions on whether using sodium thiosulfate or 95% alcohol is better for removing the iodine?
Steve
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Message: 12
Date: Fri, 17 Jun 2005 08:11:11 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Red chromogens
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BED8361F.9046%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
Just to add a quick note, I like the Dako permanent red chromogen. It is for
use with alkaline phosphatase detections. It is nice & bright, easy to use.
Great for double labeling, too.
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
------------------------------
Message: 13
Date: Fri, 17 Jun 2005 09:17:28 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] formic acid decal
To: "'Trisha Emry'" <emry <@t> u.washington.edu>, "'histo'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001401c5734f$adf76750$a7d48a80 <@t> AMY>
Content-Type: text/plain; charset="us-ascii"
Trisha
I have used 20% formic acid made up in distilled water on sheep tibias
and it worked just fine.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Trisha
Emry
Sent: Thursday, June 16, 2005 2:54 PM
To: histo
Subject: [Histonet] formic acid decal
I need to speed up the decal process on some large bone samples.
What is the highest precentage of formic acid that I can use without
damaging the bones?
I am using sodium formate with formic acid now.
Thanks,
Trisha
Seattle
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------------------------------
Message: 14
Date: Fri, 17 Jun 2005 10:28:46 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] elastic stain
To: "'Steven Coakley'" <sjchtascp <@t> yahoo.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200506171628.j5HGSgt1005594 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
I use Movat's Pentachrome as a batch stain for elastic fibers. I get the
kit from Polyscientific and reuse many of the reagents.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Friday, June 17, 2005 8:07 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] elastic stain
Good morning everyone,
Is there any elastic stain thats reliable for batch staining, that still
give the domonstration of fine/course of a good VVG? Also has anyone the
potassium permanganate/oxalic acid prior to the elastic working solution to
achieve better staining of elastic fibers? Any opinions on whether using
sodium thiosulfate or 95% alcohol is better for removing the iodine?
Steve
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------------------------------
Message: 15
Date: Fri, 17 Jun 2005 10:29:34 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Rodent mast cell IHC
To: "'Thomas Crowell'" <Thomas.Crowell <@t> biogenidec.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200506171629.j5HGTUt1005826 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
Not in my experience.
Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thomas
Crowell
Sent: Friday, June 17, 2005 7:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rodent mast cell IHC
Can anyone tell me if DakoCytomations C-kit CD117 polyclonal antibody
crosses with mouse FFPE mast cells?
Thanks
Tom Crowell
BiogenIdec
Cambridge, MA
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------------------------------
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End of Histonet Digest, Vol 19, Issue 25
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