[Histonet] Species specific antibody marker

[Jackie M O'Connor]

Affinity BioReagents, (ABR) has a Mouse MAB (vimentin) which is specific 
for Mouse, Human and Rat. (MA3-745).  You can use this to distinguish 
murine from canine cells, theoretically.  I've used a human mitochondria 
marker to distinguish human from murine - everything that stains is human 
- the ones that don't stain are murine.  This should work the same, I 
would hope.  I haven't found a murine mitochondria marker so far.
Jackie

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor <@t> abbott.com




"Colin Nixon" <csn1x <@t> udcf.gla.ac.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/17/2005 08:28 AM
Please respond to Colin Nixon

 
        To:     <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Species specific antibody marker


I have received FFPE mouse tissue that has been treated with canine MDCK 
cells. It has been established that there are carcinoma cells present in 
the mice tissue. What I am trying to establish is whether the tumour has 
originated from a canine or murine origin. Does anyone know of any 
immunohistochemical markers that would be species specific for this task?

Thanks,

Colin
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