[Histonet] Cryostat sections with small bubbles

Katri Tuomala katri <@t> cogeco.ca
Tue Jun 14 20:39:51 CDT 2005

Hi Emily,
I have never heard of hot plating cryostat sections. You may be boiling the 
moisture under the section forming bubbles or do you air dry them well 
first? Also -35 C seems too cold for brain sections, you may be getting some 
cutting artifacts.
I'm sure there'll be others with helpful information for you.

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Emily Jane Wiesner-Camm" <Emily.Wiesner <@t> medecine.unige.ch>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, June 14, 2005 10:36 AM
Subject: [Histonet] Cryostat sections with small bubbles

> Hi All,
> I was wondering whether anyone can let me know why small bubbles appear 
> underneath my rat brain sections when I cut them on a cryostat  (at -35) 
> when they appear perfectly flat before placing them on a hotplate. How 
> they can be avoided?
> Thanks,
> Emily
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