[Histonet] iron hematoxylins

[Lee & Peggy Wenk]

I've never blued after a Weigert iron hematoxylin stain. Nuclei are either
black, or they aren't. If they are not, then the iron hematoxylin is old
(more than a couple days old), and putting the tissue into an alkali
solution won't bring the color back.

I'm curious, why are you bluing? What is the purpose? Maybe then I can help.

Peggy Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Tuesday, June 14, 2005 10:49 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] iron hematoxylins

Does anyone know if using a bluing agent after weigerts iron hematoxylin
will achieve the same results a lengthy tap water rince?
 
Steve

		
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