[Histonet] polyoma virus

Van Eyck, Deb deb.vaneyck <@t> phci.org
Mon Jun 13 16:46:19 CDT 2005

Hi all,

   A question for any of you out there doing polyoma virus on urine cyto
specs----I am working up Nova Castra's JC/BVK immuno stain for acetone fixed
cell smears. (Works well on thin prep material with a 20n minute cold
acetone post fix).  In your experiences using this or other immuno
procedures are you getting staining in most of the decoy cells as well as
cytoplasmic staining in infected cells and free virus particles?  My results
are very clean and I am seeing some total nuclear staining in some of the
decoy cells, but not all,  some nuclear ring like staining, some cytoplasmic
inclusion like staining and some clumps of what I am thinking are free virus
particles?   Any one out there using SV-40 for cyto specs and polyoma virus?
If so would you please share your protocol with me? thanks very much.

This information is confidential and intended solely for the use of the
individual or entity to whom it is addressed. 
If you have received this email in error please notify the sender or our
Customer Support Center at (262) 928-2777. 

We have scanned this email and its attachments for malicious content.
However, the recipient should check this email and any attachments for the
presence of viruses.
ProHealth Care accepts no liability for any damage caused by any virus
transmitted by this email. 

More information about the Histonet mailing list