[Histonet] antigen retrieval in bone paraffin sections

Elizabeth Chlipala liz <@t> premierlab.com
Fri Jun 10 16:42:01 CDT 2005


I frequently use the ready to use proteinase K from DakoCytomation on
formalin fixed, formic acid decalcified bone sections.  I get good
results using proteinase K with antibodies such as F4/80 in mouse
joints, ED-1 in rat joints.  IL-6 in both mouse and rat joints, mouse
neutrophils, factor H, BrdU and Factor VIII.  The other retrieval method
I use is BioGenex's DeCal.  I use that for CD45R in mice.  I try to stay
away from HEIR.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pex
Sent: Friday, June 10, 2005 2:25 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval in bone paraffin sections

Hello, all,=20

Do you have some good suggestions about antigen retrieval in bone
paraffin sections?=20

Thank you!=20


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