[Histonet] ID rat capillarys in muscle
rocan <@t> mac.com
Thu Jun 9 11:49:41 CDT 2005
I would strongly suggest that you look up the papers from Donald M.
McDonald (UCSF). Search on medline for author "McDonald DM". There
absolutely no other group in the world better for vessel staining in
vivo. I do recommend you do it in vivo before euthanasia. We have
developed all our methods his papers.
In brief: you inject the animal with a very small amount (less than
1ug), of griffonia isolectin B4 conjugated to a fluorochrome or
biotin. Then you must perfuse the animal with 0.1% FRESH
payaformaldehyde in (PBS). The perfusion done at 120 mmHg via left
ventricle; the right atrium is punctured for drainage. It works like a
This procedure will have all the vessels lumens stained with
fluorescence and the perfusion prevents vascular collapse. They have
modified their techniques so it is important to start with his newest
papers and go back to older ones as needed for details. In your case
it would be great because it would also tell you if the vessels had
flow. VEGF can create hemangioma-like networks that are not perfused
Contact me directly if you need any details.
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Honigmannr <@t> cshs.org
On Jun 9, 2005, at 8:14 AM, Steven Coakley wrote:
> Working with ischemic rat hindleg injected with VEGF. I've read some
> articles recommending capillary endothelial cell identification with
> CD 31, Factor VIII and RECA-1, if anyone has experiance with any of
> these, others or similar work I'd appreciate the contact.
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