[Histonet] colloidal iron
asmith <@t> mail.barry.edu
Thu Jun 9 10:12:43 CDT 2005
Since my attachment didn't work, I am sending my protocol for Mowry's
colloidal iron as text.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
Mowry, R.W. (1958) Improved procedure for the staining of acidic
polysaccharides by Muller's colloidal (hydrous) ferric oxide
and its combination with the Feulgen and periodic acid-Schiff
reactions. J. Clin. Invest. -7-: 566-576.
Stock "solution" (actually a colloidal suspension):
6.75 g FeCl3.6H2O
6 ml distilled water
0.4 ml conc. (37%) HCl
Stir until dissolved.
Bring 250 ml distilled water to a boil in a 1 liter container.
Add ferric chloride solution to the boiling water.
15 ml distilled water
5 ml glacial acetic acid
20 ml stock "solution"
15 ml 2% potassium ferrocyanide trihydrate
[0.3 g K4Fe(CN)6.H2O in 15 ml H2O]
Make 30 minutes before use.
15 ml 2% solution of conc. (37%) HCl
[0.3 ml 37% HCl plus 14.7 ml H2O]
Mix solutions just before use.
1 N HCl:
27.5 ml H2O
2.5 ml conc. (37%) HCl
0.5 g sodium pyrosulfite = "sodium metabisulfite" = Na2S2O5
100 ml distilled water
0.5 ml conc. (37%) HCl
1. Bring to water.
2. 12% acetic acid, 30 seconds.
3. Colloidal iron, 1 hour.
4. 12% acetic acid, 4 changes, 3 minutes each.
5. Ferrocyanic acid, 20 minutes.
6. Running tap water, 5 minutes.
7. 1 N HCl at 60oC, 10 minutes.
8. Running tap water, 5 minutes.
9. Distilled water, rinse.
10. Schiff's reagent, 10 minutes.
11. Sulfurous acid, 3 changes, 2 minutes each.
12. Running water, 5 minutes.
13. Dehydrate, clear, and mount.
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)
More information about the Histonet