[Histonet] colloidal iron

Smith, Allen asmith <@t> mail.barry.edu
Thu Jun 9 10:12:43 CDT 2005

Since my attachment didn't work, I am sending my protocol for Mowry's
colloidal iron as text.


Allen A. Smith, Ph.D.

Professor of Anatomy

Barry University School of Graduate Medical Sciences

    Podiatric Medicine and Surgery

Miami Shores, Florida  33161




                        COLLOIDAL IRON

Mowry, R.W. (1958)  Improved procedure for the staining of acidic

    polysaccharides by Muller's colloidal (hydrous) ferric oxide

    and its combination with the Feulgen and periodic acid-Schiff

    reactions.  J. Clin. Invest. -7-: 566-576.


Stock "solution" (actually a colloidal suspension):

    6.75 g FeCl3.6H2O

    6 ml distilled water

    0.4 ml conc. (37%) HCl

  Stir until dissolved.

  Bring 250 ml distilled water to a boil in a 1 liter container.

  Add ferric chloride solution to the boiling water.



Colloidal iron:

    15 ml distilled water

     5 ml glacial acetic acid

    20 ml stock "solution"


Ferrocyanic acid:

    15 ml 2% potassium ferrocyanide trihydrate

           [0.3 g K4Fe(CN)6.H2O in 15 ml H2O]


           Make 30 minutes before use.

    15 ml 2% solution of conc. (37%) HCl

           [0.3 ml 37% HCl plus 14.7 ml H2O]


    Mix solutions just before use.


1 N HCl:

    27.5 ml H2O

     2.5 ml conc. (37%) HCl


Sulfurous acid:

    0.5 g sodium pyrosulfite = "sodium metabisulfite" = Na2S2O5

  100 ml distilled water

    0.5 ml conc. (37%) HCl


 1. Bring to water.

 2. 12% acetic acid, 30 seconds.

 3. Colloidal iron, 1 hour.

 4. 12% acetic acid, 4 changes, 3 minutes each.

 5. Ferrocyanic acid, 20 minutes.

 6. Running tap water, 5 minutes.

 7. 1 N HCl at 60oC, 10 minutes.

 8. Running tap water, 5 minutes.

 9. Distilled water, rinse.

10. Schiff's reagent, 10 minutes.

11. Sulfurous acid, 3 changes, 2 minutes each.

12. Running water, 5 minutes.

13. Dehydrate, clear, and mount.





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