[Histonet] MACCHIAVELLO STAIN

[Barbara Bublava]

Hi all

I am also unable to get my methylene blue to work. Different solutions,
different concentrations with and without acidic acid, with and without
postfixation in bouin ...
(trying to do a Masson)

Whats going wrong?

----- Original Message ----- 
From: <Secholsb <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 08, 2005 2:34 AM
Subject: [Histonet] MACCHIAVELLO STAIN


> Has anybody done a macchiavello stain for chlamydia?  If so,  HELP....
> I am unable to get my methylene blue to stain my background.   I can leave
> the slides in methylene blue over night and it still will not  stain.
What
> could I possibly
>  be doing incorrectly?  Thanks
>
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