[Histonet] State requirements for an HT
DEllenburg2 <@t> stfrancishealth.org
DEllenburg2 <@t> stfrancishealth.org
Mon Jun 6 13:07:18 CDT 2005
As far as I know we do not have any State regulations requiring an
individual to be certified to work in the histology dept. However,
there is a difference in the pay grade for certified and non-certified
techs. We currently have one certified and 2 non-certified techs.
Hope this helps.
Debbie Ellenburg
Supervisor
Bon Secours St. Francis Health System
864-255-1582
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Subject: Histonet Digest, Vol 19, Issue 9
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Today's Topics:
1. RE: Vision Bio Systems (Barry Madigan)
2. Re: CD4, CD8 and Bcells (antje.marcantonio <@t> novartis.com)
3. skeletal muscle regeneration marker? (Baowei Peng)
4. Processing GMA Specimens (Snider, Deanna)
5. RE: Tissue Microarrays. (Thom Jensen)
6. State requirements for HT (Thweatt, John T)
7. RE: Tissue microarrays use?. . (Henry, Charlene)
8. FW: [Histonet] Tissue microarrays use?. . (Patti Loykasek)
9. RE: Saturday work schedule (Bonner, Janet)
10. RE: Saturday work schedule (Bonner, Janet)
11. Qualifications to work in Histology
(Frederick.Fifield <@t> sunhealth.org)
12. Re: Cutting undecalcified bone (Instrumedics)
----------------------------------------------------------------------
Message: 1
Date: Mon, 6 Jun 2005 09:02:16 +1000
From: "Barry Madigan" <madbaza <@t> powerup.com.au>
Subject: RE: [Histonet] Vision Bio Systems
To: "'Terry Murphy'" <lubbockcat <@t> hotmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20050605230216.TTMS16131.smta05.mail.ozemail.net <@t> WORKSTATION1>
Content-Type: text/plain; charset="us-ascii"
Hello Terry
We have two of Visions Bond Immunostainers and a number of their Peloris
tissue processors.
Have had the Bonds for about 18 months now and the tissue processors almost
a year.
We have been very pleased with their service.
Just send me an email on the info you require.
Regards
Barry Madigan
Immunohistochemistry
Queensland Health Pathology Services (QHPS)
Royal Brisbane Hospital
Australia
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Terry Murphy
Sent: Saturday, 4 June 2005 9:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Vision Bio Systems
Does anyone on the histonet have any experience with Vision BioSystems. I
am looking for any information from anyone who has dealt with the company
and it's products, good or bad. Thank you
Terry Murphy
HTL (ASCP)
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------------------------------
Message: 2
Date: Mon, 6 Jun 2005 08:39:03 +0200
From: antje.marcantonio <@t> novartis.com
Subject: Re: [Histonet] CD4, CD8 and Bcells
To: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFB4EC8FFF.5DD6DF75-ONC1257018.00234ACA-C1257018.002488F2 <@t> EU.novartis.net>
Content-Type: text/plain; charset="us-ascii"
Noelle,
for B cells in rat FFPE sections we use the mouse anti-rat CD45R from
Pharmingen. AR with citrate buffer needed.
The antibody works well for us with a dilution of 1/5000 and overnight
incubation.
For CD4 (Serotec MCA55G) and CD8 (Serotec MCA48G) we use frozens.
According to the datasheets these antibodies can be used as well for
paraffin-embedded material after PLP fixation.
Hope this helps somehow.
Cheers,
Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
Tel. +41 61 324 6730
antje.marcantonio <@t> novartis.com
------------------------------
Message: 3
Date: Mon, 6 Jun 2005 16:19:45 +0800 (BEIST)
From: Baowei Peng <pengbw <@t> sjtu.edu.cn>
Subject: [Histonet] skeletal muscle regeneration marker?
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050606081945.4966611139CF <@t> sjtu.edu.cn>
Content-Type: text/plain; charset="gb2312"
Hi to all,
Does anyone know if there is a marker for skeletal muscle regeneration,
especialy for myoblast? Which company can I get it?
Thanks in advance!
Baowei Peng
1954 Huashan Road
Pharmacy School
Shanghai Jiaotong University
Shanghai, 200030
China
Ph:86-21-62932108
E-mail:pengbw <@t> sjtu.edu.cn
------------------------------
Message: 4
Date: Mon, 6 Jun 2005 10:01:29 -0400
From: "Snider, Deanna" <dsnider <@t> shrinenet.org>
Subject: [Histonet] Processing GMA Specimens
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A505C26A8103D511B3CE00306E06479102AB239E <@t> CINFS4>
Content-Type: text/plain; charset="ISO-8859-1"
Hello again everyone!
You have all been very helpful and I need (yet again) to gather more
information from everyone! As previously stated, I have little knowledge
working with GMA specimens. It has been a learning experience for me!
I process clinically engeineered skin samples. They are very small and
thin. The program I use, (which was programmed into the RMC, EMP5160 when I
took the job) is almost 5 hours long. The last 3 steps are 100% GMA
preperation solution, each an hour long. After reviewing other protocols,
this seems quite long for the samples I am working with. I would appreciate
any protocols or suggestions you may have. You can email or fax them to me!
Again, ALL help is greatly appreciated, and thank you in advance!
Deanna Snider HT ASCP
Shriners Hospital for Children
Research Dept.
Cincinnatti, Oh
513-872-6388
513-872-6072 (fax)
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------------------------------
Message: 5
Date: Mon, 06 Jun 2005 14:55:34 +0000
From: "Thom Jensen" <tissuearray <@t> hotmail.com>
Subject: RE: [Histonet] Tissue Microarrays.
To: MadaryJ <@t> MedImmune.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY102-F3357F53532C3C4B615692DBBFB0 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"
Tissue Microarrays are not hard to do once you understand the
process. I have several articles on constructing microarrays and I
answer questions in email and phone often. You can go the my website
and see a basic array created without using expensive instruments and
other helpful information.
arrayworkshop.com
I hope this helps
Thom Jensen
>From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Tissue Microarrays.
>Date: Fri, 3 Jun 2005 16:09:54 -0400
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>
>What kind of response did you get on this? I have a pathologist who
seems real hung up on contracting out TMA's to someone (actually in
England, maybe you!). I am justm wondering from a routine standpoint
and doing some pre-clinical work on rodents, what would be the benefit
of us getting a system in place or contracting out this work? I mean
I took a class in how to prepare them a few years back, bascially
taking the specimens and putting them in spots on the block with the
legend etc. I mean could one do this without fancy machinery, and
what is the real use for this stuff?
>
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 6 Jun 2005 08:00:43 -0700
From: "Thweatt, John T" <John.Thweatt <@t> med.va.gov>
Subject: [Histonet] State requirements for HT
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7A00E2723D55D311AC240000F831866802606F57 <@t> vhaamaexc1.amarillo.med.va.gov>
Content-Type: text/plain; charset="iso-8859-1"
Dear Fellow Histonetters,
Does anyone know or heard of any "State" requirements for Histotechs to
work in the lab setting without certification?
Thanks in advance,
Tom Thweatt
------------------------------
Message: 7
Date: Mon, 6 Jun 2005 10:22:11 -0500
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
Subject: RE: [Histonet] Tissue microarrays use?. .
To: "Katri Tuomala" <katri <@t> cogeco.ca>, "Madary, Joseph"
<MadaryJ <@t> MedImmune.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5CB39BCA5724F349BCB748675C6CA1A202C7572C <@t> SJMEMXMB02.stjude.sjcrh.local>
Content-Type: text/plain; charset="us-ascii"
Our pathologist review H&E slides of each block to be used in a tissue
array and they determine which blocks are grouped together for each
tissue array block. They also mark on the H&E slide the exact area they
want punched.
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: Katri Tuomala [mailto:katri <@t> cogeco.ca]
Sent: Friday, June 03, 2005 5:12 PM
To: Henry, Charlene; Madary, Joseph; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue microarrays use?. .
Hi Charlene,
Knowing that so many tumors can give a very heterogenous staining
patterns
( some areas strongly positive and others weak or even negative ), how
do
you overcome this problem with microarrays? I would have a hard time
trusting the results with such small samples.
Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
To: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 03, 2005 4:43 PM
Subject: RE: [Histonet] Tissue microarrays use?. .
They are greatly instrumental in research. Working at a research
facility, we were able to pay for the purchase of the instrument with
only 1 research project. Example: A pathologist has a research project
that he/she is doing say on Neuroblastoma. They have 150 blocks that
they need a total of 10 IHC tests on each block. You take the 150 blocks
and prepare 2 tissue micro array blocks and then run your 10 IHC test on
the 2 TMA blocks. You have saved a great deal of money because you have
20 IHC tests instead of 1500 IHC tests that would have been needed
without the tissue array blocks. At approximately $15 for each IHC test,
you can see that $300 is much better than $22,500.
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
Joseph
Sent: Friday, June 03, 2005 3:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue microarrays use?. .
Can someone explain to me what the big deal is on tissue microarrays? I
understand that they are many perfect circles of known diseased or
normal tissues than can be used as a control for various applications.
What are the applications for this stuff on routine or research that
would make it worth it for us to either do it ourselves or contract it
out? Can it be done in house cheap?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 8
Date: Mon, 06 Jun 2005 08:48:07 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: FW: [Histonet] Tissue microarrays use?. .
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BEC9BE47.8E02%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
I think everyone has had very good comments about the use of microarrays,
and I agree with all of them. I would like to add, that it is the usual
practice to put more than 1 core from a block into an array. This somewhat
addresses the problem with heterogeneity, but doesn't totally solve it.
Just my 2 cents.
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
------ Forwarded Message
From: "Katri Tuomala" <katri <@t> cogeco.ca>
Date: Fri, 3 Jun 2005 18:11:51 -0400
To: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>, "Madary, Joseph"
<MadaryJ <@t> MedImmune.com>, <histonet <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] Tissue microarrays use?. .
Hi Charlene,
Knowing that so many tumors can give a very heterogenous staining patterns
( some areas strongly positive and others weak or even negative ), how do
you overcome this problem with microarrays? I would have a hard time
trusting the results with such small samples.
Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
To: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 03, 2005 4:43 PM
Subject: RE: [Histonet] Tissue microarrays use?. .
They are greatly instrumental in research. Working at a research
facility, we were able to pay for the purchase of the instrument with
only 1 research project. Example: A pathologist has a research project
that he/she is doing say on Neuroblastoma. They have 150 blocks that
they need a total of 10 IHC tests on each block. You take the 150 blocks
and prepare 2 tissue micro array blocks and then run your 10 IHC test on
the 2 TMA blocks. You have saved a great deal of money because you have
20 IHC tests instead of 1500 IHC tests that would have been needed
without the tissue array blocks. At approximately $15 for each IHC test,
you can see that $300 is much better than $22,500.
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
Joseph
Sent: Friday, June 03, 2005 3:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue microarrays use?. .
Can someone explain to me what the big deal is on tissue microarrays? I
understand that they are many perfect circles of known diseased or
normal tissues than can be used as a control for various applications.
What are the applications for this stuff on routine or research that
would make it worth it for us to either do it ourselves or contract it
out? Can it be done in house cheap?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------ End of Forwarded Message
------------------------------
Message: 9
Date: Mon, 6 Jun 2005 11:48:10 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] Saturday work schedule
To: "'jmy1967 <@t> comcast.net'" <jmy1967 <@t> comcast.net>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB42B3 <@t> fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
We have approximately the same workload and have two techs and a Lab Aide.
One tech comes in around 1AM and embeds biopsies and as much Sat. work as
possible, cuts the blocks and sp. stains if she has the time. The second
Tech comes in around 3-4AM and cuts, does the special stains, and embeds the
Monday work (we process all our tissue on Friday night and embed it Sat
incase the Pathologist decides he wants a case slated for Monday). The Lab
Aide comes in around 4-6AM and checks to make sure we have all the slated
Sat. work, labels the slides, distributes the slides to the two Saturday
Pathologists, and cleans up the stainer and processors when all the slides
redistributed. The second tech is working until noon, and then we have an
on-call person from noon until 4PM, and on Sunday on-call from 10AM to 4PM.
We have a separate immuno dept. (- we do about 300,000 slides per year).
Hope this helps - Janet
--Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
jmy1967 <@t> comcast.net
Sent: Friday, June 03, 2005 7:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Saturday work schedule
Dear Histonetters,
We are considering adjusting our Saturday work schedule. Currently, two
techs embed about 130 biopsy blocks and section and stain three slides for
each. I'd like some information on how other high volume labs implement
their Saturday schedule. Do you have a Saturday rotation? And if so, what's
your work flow protocol, i.e. number of techs, type of specimens produced
(biopsies and/or surgicals)? If your lab has two or more shifts, is the
Saturday rotation equitable or does only day shift rotate on Saturdays? Many
thanks for your responses!
Jill
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 6 Jun 2005 12:04:58 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] Saturday work schedule
To: "'jmy1967 <@t> comcast.net'" <jmy1967 <@t> comcast.net>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB42B4 <@t> fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
I neglected the rest of your question! About the shift rotation. I'm the
latest shift that applies to working Saturday - 10AM to 6:30PM. Obviously I
have to take Fridays off to get up at 2AM on Saturday to make it by 3AM(I'm
then the late Saturday person - until 12 noon) The next shift that does not
apply to the Saturday rotation comes in at 3PM-11:30PM. The majority of our
people work 10PM-6:30AM and 6AM-2:30PM. This shift covers early Saturdays
and has Mondays off. (By "shift", we actually have our own hours skewed
throughout the day, ie- I'm the only 10AMer.)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
jmy1967 <@t> comcast.net
Sent: Friday, June 03, 2005 7:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Saturday work schedule
Dear Histonetters,
We are considering adjusting our Saturday work schedule. Currently, two
techs embed about 130 biopsy blocks and section and stain three slides for
each. I'd like some information on how other high volume labs implement
their Saturday schedule. Do you have a Saturday rotation? And if so, what's
your work flow protocol, i.e. number of techs, type of specimens produced
(biopsies and/or surgicals)? If your lab has two or more shifts, is the
Saturday rotation equitable or does only day shift rotate on Saturdays? Many
thanks for your responses!
Jill
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Mon, 6 Jun 2005 09:11:09 -0700
From: Frederick.Fifield <@t> sunhealth.org
Subject: [Histonet] Qualifications to work in Histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFD25E847C.53CA5FB3-ON07257018.00563EB4-07257018.0059402B <@t> SunHealth.org>
Content-Type: text/plain; charset=US-ASCII
Hello Tom
I'm in Arizona and as far as I know there are no state regulations
regarding the certification of histotechs, nor does my facility have any
requirements in that regard although we give preference to those who are
certified in those situations when we have a certified and non-certified
applicant for an opening. Most labs where I have worked have non-certified
techs. At the lab that I currently manage we have two certified techs and
two non-certified techs. The non-certified techs are in the process of
getting their certification.
Most facilities in Phoenix would prefer that their techs are certified and
certified techs will usually be hired before non-certified ones. Since
there is no state requirement many non-certified techs in Arizona have
never felt it necessary to challenge the exam because in the long run it
has no effect on their pay or their ability to be hired. Our non-certified
techs receive the same pay that a certified tech would receive. I have
questioned this with our HR department and was told that wages are based on
the ability to do the job and not of certification. If and when my
non-certified techs become HT's they will not receive any additional pay
for being certified.
As long as non-certified techs have the ability to be hired and receive the
same wages as certified techs this situation is not like to change soon in
this area. I hope this helps to answer your questions.
Fred Fifield BS, HT (ASCP)
Pathology Section Manager
Sun Health - Boswell Memorial Hospital
(623) 876-5338
Dear Fellow Histonetters,
Two posts in one day ought to be a record for me, but as demand
dictates, I have returned with yet another question.
This question may be directed more toward the Histology managers or
histologists who know a little about regulations in the group as I once
again as for your most valuable help.
I am inquiring on behalf of a friend and fellow Histologist who at the
moment does not have access to a computer, so pardon me if this isn't very
clear as this is coming to me second hand.
The question is: What are the individual state requirements for a
histology technician that is not certified? Is there such regulation that
exists within the states that would regulate hiring staff and what kind of
requirements would a person fall under in order to be able to be hired to
work in histology (non-certified). What does your state say about this
issue?
I would be interested to see what different states (or facilities)
have
to say about the uncertified technician route as it got a little harder now
to get certified under ASCP.
If anyone has any facility type documentation that gives you guidance
as to who you can hire to work in histology, what kind of qualifications do
you require? Does your state or facility eventually push for certification
(surely)? Are there cities out there that lay the groundwork for what
qualifications are necessary to work in our field?
I do realize that this is a complex question that may not a simple
answer. If someone out there has the expertise in this could give me some
guidance on this topic it would be most appreciated. Thank you in advance.
Sincerely,
Tom Thweatt
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Message: 12
Date: Mon, 6 Jun 2005 12:34:39 -0400
From: "Instrumedics" <info <@t> instrumedics.com>
Subject: Re: [Histonet] Cutting undecalcified bone
To: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Cc: HistoNet Server <HistoNet <@t> Pathology.swmed.edu>
Message-ID: <008201c56ab5$ad674be0$6401a8c0 <@t> INSTRUMEDICS22>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Patsy,
I know from experience that many bone labs with the CryoJane system have
difficulty in getting "perfect" sections and transferring them fully
intact. However, many of them seem to find a technique for success.
Dr. Rowe and his colleagues at UConn have been very successful with
tape-transfer in cutting decalcified and undecalcified bone. I believe he
has given a workshop(s) on his method.
Perhaps you can contact Dr. Rowe's lab.
Bernice
Instrumedics
----- Original Message -----
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: "'Stephen Peters M.D.'" <petepath <@t> yahoo.com>;
<Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 03, 2005 5:38 PM
Subject: RE: [Histonet] Cutting undecalcified bone
>I have experience cutting frozen sections of whole rat tibias without decal
> (John Tarpley and I did this work together) which in my experience could
> only be done using the tape transfer technique. I use a D profile
> tungsten
> carbide knife(the bevel is only on one side unlike a C profile which has
> the
> same bevel on both sides, this is like the old triangle shaped glass
> knives)
> this knife requires a pretty straight cutting angle compared to the C
> profile which is more angled, thus the reference Liz had about me changing
> the knife angle. I have tried everything from heavily coated 6X to the
> lightest 1/2X coated slides. I have still to find a technique which will
> give me perfect sections of bone everytime. The problem for me is not
> getting the perfect section cut but getting it attached to the coated
> slide
> without bubbles under the tape and then removing the tape without leaving
> some of the section on the tape. This can be very frustrating and
> expensive
> to cut a beautiful section only to have it pull off with the tape, the
> expensive slide is lost and your beautiful section is useless.
> This is not only the case for undecalcified bone, I have been having the
> same old problem with cornea implants. Some things to try include: try
> to
> cut a thinner section like 4 microns (if your knife is really sharp this
> is
> not a problem), the thinner section will lay flatter on the coated slide.
> I
> do expose the UV for 2-3 times with about 10 secs. Between or the fuse
> will
> blow. Pull the tape off diagnally from corner to corner very slowly and
> smoothly. When appling the tape with the section to the coated slide
> "role
> the hell out of it" to borrow a phrase from Gayle, to make sure it gets
> really well attached to the slide before UV. I have had some luck with
> preparing the slide, exposing it to UV and then placing it on dryice for
> 5-10 min. before removing the tape, but again I never get every section in
> good shape and actually lately I have been getting more bad sections than
> good, which is driving me crazy. There are people up at CSU using this to
> cut horse carpal bone, I can give you their contact info if you like. I
> think I did better with bone than I am doing with cornea right now.
> Oh, for bone I try to cut as cold as possible, about -35 I have been
> cutting
> the softer tissue warmer -18---24, hey maybe that's my problem I should go
> back to the cold cold bone method, it can't hurt at this point.
> I snap freeze samples by surrounding them with OCT and either slowly
> lowering into liq. Nitrogen or isopentane and dryice.
> Patsy
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephen
> Peters M.D.
> Sent: Thursday, June 02, 2005 10:18 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cutting undecalcified bone
>
> Nancy,
>
> I have experience cutting frozens on undecacified bone specimens for
> routine
> surg path cases using conventional technique but have no experience with
> the
> tape transfer system.
> I routinely use disposible low profile blades which in not optimum for
> bone. I imagine a stronger high profile blade or knife will work better.
> My
> success has varied with the hardness of the bone. Trebecular bone coming
> from older patients can cut fairly easily porvided the blade is sharp, and
> the section is taken with a fluid continuous motion. The result is a
> surprisingly intact section. The harder the bone ( typically the younger
> the
> patient ) there will be more damage to the blade in each pass. The
> sections
> will be streaked and splitting requiring one or more blade changes by time
> I
> get a resonable section. It is quite a juggling act.
> Cutting hard cortical bone is often very frustrating and very difficult to
> get any resonable section. From what you describe as bubbles, I am
> guessing
> you are getting sections of bone thicker than you are hoping for and as a
> result when you roll it out, the thicker bone pieces are protecting the
> rest
> of the section from rolling. Often the first section of any tissue will be
> considerably thicker than those that follow after a few fluid turns of the
> wheel. If you are not letting a few pass your first section may be
> considerably thicker and as a result will shatter more and create thicker
> trabeculae which may be leading to your problem.
> The equivalent using conventional technique will give me a section that my
> coverslip will not lie flat against the slide and my mounting medium will
> not spread.
> I look foreward to other peoples experience on this subject.
>
> Stephen
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
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End of Histonet Digest, Vol 19, Issue 9
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