[Histonet] CD4, CD8 and Bcells

antje.marcantonio <@t> novartis.com antje.marcantonio <@t> novartis.com
Mon Jun 6 01:39:03 CDT 2005


for B cells in rat FFPE sections we use the mouse anti-rat CD45R from 
Pharmingen. AR with citrate buffer needed.
The antibody works well for us with a dilution of 1/5000 and overnight 
For CD4 (Serotec MCA55G) and CD8 (Serotec MCA48G) we use frozens. 
According to the datasheets these antibodies can be used as well for 
paraffin-embedded material after PLP fixation.
Hope this helps somehow.


Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
Tel. +41 61 324 6730
antje.marcantonio <@t> novartis.com

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